Microalgal transformation of progesterone by the terrestrial-isolated cyanobacterium Microchaete tenera

A large number of microorganisms including various microalgal strains are able to convert steroid compounds into useful metabolites. In the present study, the ability of Microchaete tenera, a rice paddy field-isolated microalga, was investigated for biotransformation of progesterone. The incubation...

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Bibliographic Details
Published in:Journal of applied phycology 2012-08, Vol.24 (4), p.777-781
Main Authors: Safiarian, Mohammad Sadegh, Faramarzi, Mohammad Ali, Amini, Mohsen, Soltani, Neda, Tabatabaei-Sameni, Minoosadat, Hasan-Beikdashti, Morvarid
Format: Article
Language:English
Subjects:
Biomedical and Life Sciences
biotransformation
carbon dioxide
Cyanobacteria
Ecology
Fourier transform infrared spectroscopy
Freshwater & Marine Ecology
Life Sciences
lighting
metabolites
microalgae
Microchaete tenera
microorganisms
nuclear magnetic resonance spectroscopy
Oryza sativa
Plant Physiology
Plant Sciences
progesterone
rice
spectral analysis
steroids
temperature
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Summary:A large number of microorganisms including various microalgal strains are able to convert steroid compounds into useful metabolites. In the present study, the ability of Microchaete tenera, a rice paddy field-isolated microalga, was investigated for biotransformation of progesterone. The incubation was carried out at 25°C under continuous illumination in the present of 0.25 g L−1 of progesterone. After 5 days incubation of the microalga in BG-11 liquid medium, the broth was extracted and the products were purified by the aid of chromatographic methods. Structure elucidation of the metabolites was performed by spectral data (13C NMR, 1H NMR, FTIR, and MS) and physical constants (melting points and optical rotations). Eventually, four major steroids including 20β-hydroxypregn-4-en-3-one, 20α-hydroxypregn-4-en-3-one, 6β-hydroxypregn-4-en-3,20-dione and 6α-hydroxypregn-4-en-3,20-dione were the results of this biotransformation. The study also showed that the best concentration of starting material, temperature, photoregime, and the influence of CO2 partial pressure on the production of bioconverted metabolites were 0.25 g L−1, 25°C, continuous light and 2.0 ± 0.1% (v/v), respectively. Highest concentrations of all biotransformed metabolites were obtained in the 5th day.
ISSN:0921-8971
1573-5176
DOI:10.1007/s10811-011-9697-9