Purification and Functional Reconstitution of a Seven-Subunit Mrp-Type Na+/H+ Antiporter

• Published in: Journal of Bacteriology 2014-01, Vol.196 (1), p.28-35
• Main Authors: Morino, Masato, Suzuki, Toshiharu, Ito, Masahiro, Krulwich, Terry Ann
• Format: Article
• Language: English
• Subjects: Adenosine triphosphatase; adenosine triphosphate; adenosinetriphosphatase; Bacillus; Bacillus - enzymology; Bacillus pseudofirmus; Bacteria; Bacteriology; Cytoplasm; H-transporting ATP synthase; Homeostasis; hydrophobicity; Liposomes - chemistry; Liposomes - metabolism; Membranes; Protein Multimerization; Protein Subunits - isolation & purification; Protein Subunits - metabolism; Proton-Motive Force; purification methods; sodium; Sodium - metabolism; sodium-hydrogen antiporter; Sodium-Hydrogen Exchangers - isolation & purification; Sodium-Hydrogen Exchangers - metabolism
• ISSN: 0021-9193; 1098-5530; 1067-8832
• DOI: 10.1128/JB.01029-13

Mrp antiporters and their homologues in the cation/proton antiporter 3 family of the Membrane Transporter Database are widely distributed in bacteria. They have major roles in supporting cation and cytoplasmic pH homeostasis in many environmental, extremophilic, and pathogenic bacteria. These antiporters require six or seven hydrophobic proteins that form hetero-oligomeric complexes, while most other cation/proton antiporters require only one membrane protein for their activity. The resemblance of three Mrp subunits to membrane-embedded subunits of the NADH:quinone oxidoreductase of respiratory chains and to subunits of several hydrogenases has raised interest in the evolutionary path and commonalities of their proton-translocating domains. In order to move toward a greater mechanistic understanding of these unusual antiporters and to rigorously demonstrate that they function as secondary antiporters, powered by an imposed proton motive force, we established a method for purification and functional reconstitution of the seven-subunit Mrp antiporter from alkaliphilic Bacillus pseudofirmus OF4. Na+/H+ antiporter activity was demonstrated by a fluorescence-based assay with proteoliposomes in which the Mrp complex was coreconstituted with a bacterial FoF1-ATPase. Proton pumping by the ATPase upon addition of ATP generated a proton motive force across the membranes that powered antiporter activity upon subsequent addition of Na+.