In vitro removal of ochratoxin A by two strains of Saccharomyces cerevisiae and their performances under fermentative and stressing conditions

• Published in: Journal of applied microbiology 2014-01, Vol.116 (1), p.60-70
• Main Authors: Petruzzi, L., Bevilacqua, A., Baiano, A., Beneduce, L., Corbo, M.R., Sinigaglia, M.
• Format: Article
• Language: English
• Subjects: binding; Biological and medical sciences; Biotechnology; DAP addition; Ethanol - metabolism; Fermentation; Fundamental and applied biological sciences. Psychology; Glycerol - metabolism; Humans; Methods. Procedures. Technologies; Microbial engineering. Fermentation and microbial culture technology; Microbiology; Molecular Sequence Data; ochratoxin A; Saccharomyces cerevisiae; Saccharomyces cerevisiae - metabolism; sugar; temperature; Toxins; Vitis - metabolism; Wine; Yeast
• ISSN: 1364-5072; 1365-2672
• DOI: 10.1111/jam.12350

Aims The aim of this research was to study the effect of time, temperature, sugar content and addition of diammonium phosphate (DAP) on ochratoxin A (OTA) removal by two strains of Saccharomyces cerevisiae using a completely randomized design. Methods and Results The strains were grown in a medium containing OTA (2 μg l−1), two sugar levels (200 and 250 g l−1), with or without DAP (300 mg l−1), and incubated at 25–30°C. The yeasts were able to decrease the toxin amount by c. 70%, with the highest removing effect observed after 3 days at 30°C in the presence of 250 g l−1 of sugars and with DAP; after 10 days, the toxin was partially released into the medium. The strains produced high ethanol and glycerol contents, showed high tolerance to single/combined stress conditions and possessed β‐d‐glucosidase, pectinase and xylanase activities. Conclusions Ochratoxin A removal was affected by time, temperature, sugar and addition of DAP. Moreover, the phenomenon was reversible. Significance and Impact of the Study Ochratoxin A removal could be an interesting trait for the selection of promising strains; however, the strains removing efficiently the toxin could release it back; thus, the selection of the starter should take into account both the removal and the binding ability of OTA.