Allelic diversity and molecular characterization of puroindoline genes in five diploid species of the Aegilops genus

Grain hardness is an important quality trait in wheat. This trait is related to the variation in, and the presence of, puroindolines (PINA and PINB). This variation can be increased by the allelic polymorphism present in the Aegilops species that are related to wheat. This study evaluated allelic Pi...

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Bibliographic Details
Published in:Journal of experimental botany 2013-11, Vol.64 (16), p.5133-5143
Main Authors: Cuesta, Susana, Guzmán, Carlos, Alvarez, Juan B
Format: Article
Language:English
Subjects:
Aegilops
Alleles
Amino Acid Sequence
Amino acids
Biological and medical sciences
breeding
Diploidy
Fundamental and applied biological sciences. Psychology
Genetic mutation
Genetic Variation
Genomes
Grains
Hardness
Molecular Sequence Data
mutation
Plant physiology and development
Plant Proteins - chemistry
Plant Proteins - genetics
Plant Proteins - metabolism
Poaceae - chemistry
Poaceae - classification
Poaceae - genetics
Poaceae - metabolism
Proteins
RESEARCH PAPER
Sequence Alignment
Species
Triticum aestivum
Wheat
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Summary:Grain hardness is an important quality trait in wheat. This trait is related to the variation in, and the presence of, puroindolines (PINA and PINB). This variation can be increased by the allelic polymorphism present in the Aegilops species that are related to wheat. This study evaluated allelic Pina and Pinb gene variability in five diploid species of the Aegilops genus, along with the molecular characterization of the main allelic variants found in each species. This polymorphism resulted in 16 alleles for the Pina gene and 24 alleles for the Pinb gene, of which 10 and 17, respectively, were novel. Diverse mutations were detected in the deduced mature proteins of these alleles, which could influence the hardness characteristics of these proteins. This study shows that the diploid species of the Aegilops genus could be a good source of genetic variability for both Pina and Pinb genes, which could be used in breeding programmes to extend the range of different textures in wheat.
ISSN:0022-0957
1460-2431
DOI:10.1093/jxb/ert299