MiR-143 induces expression of AIM2 and ASC in Jurkat cell line

Absent in Melanoma 2 (AIM2) is an intracellular microbial dsDNA sensor which plays an important role in production of proinflammatory cytokines through Apoptosis associated Speck-like protein containing a Caspase activation and recruitment domain (ASC) and Caspase-1. Micro-RNAs (miRNAs) play importa...

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Bibliographic Details
Published in:Iranian journal of immunology 2013-06, Vol.10 (2), p.103-109
Main Authors: Momeni, Mohammad, Reza Mirzaei, Mohammad, Zainodini, Nahid, Hassanshahi, Gholamhossein, Arababadi, Mohammad Kazemi
Format: Article
Language:English
Subjects:
Apoptosis
CARD Signaling Adaptor Proteins
Cell culture
Cytoskeletal Proteins - genetics
Cytoskeletal Proteins - metabolism
DNA Repair
DNA-Binding Proteins
Enzymes
Gene expression
Gene Expression Regulation, Neoplastic - genetics
Humans
Inflammasomes - immunology
Jurkat Cells
Microbiology
MicroRNAs - genetics
Nuclear Proteins - genetics
Nuclear Proteins - metabolism
Rodents
Software
Statistical methods
Studies
Up-Regulation
Viral infections
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Summary:Absent in Melanoma 2 (AIM2) is an intracellular microbial dsDNA sensor which plays an important role in production of proinflammatory cytokines through Apoptosis associated Speck-like protein containing a Caspase activation and recruitment domain (ASC) and Caspase-1. Micro-RNAs (miRNAs) play important roles in regulation of immune related genes. However, there is little information regarding the effects of miRNAs on the AIM2 and ASC expression. To determine the mRNA levels of AIM2 and ASC in Jurkat cell line following introducing miRNA-143 (miR-143). MiR-143, a scrambled sequence and PBS were introduced separately, to the Jurkat cell lines and the mRNA levels of AIM2 and ASC were examined in parallel with beta-actin and GAPDH (as housekeeping genes) using Real-Time PCR technique. The mRNA levels of AIM2 and ASC were significantly increased in the miR-143 transfected Jurkat cells when compared to the scrambled sequence or PBS treated cells. miR-143 can lead to increased expression of AIM2 and ASC mRNAs. Considering the significance of AIM2 and ASC in DNA sensing and inflammosome formation, it can be considered as a therapeutic agent for the treatment of chronic infectious diseases, especially viral infections.
ISSN:1735-1383
1735-367X