Genetic/transgenic conditional expression of full-length and headless nonmuscle myosin-II molecules: Head domain regulates localization in auditory neurons

Abstract Objective Human genetic mutations that affect the N-terminal head-domain of the nonmuscle myosin-II (MyoII) molecule can result in nonsyndromic sensorineural hearing loss but the underlying mechanism is unknown. Ultimately, MyoII must be appropriately localized in order to execute endogenou...

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Bibliographic Details
Published in:International journal of pediatric otorhinolaryngology 2013-05, Vol.77 (5), p.785-791
Main Author: Guthrie, O’neil W
Format: Article
Language:English
Subjects:
Animals
Animals, Genetically Modified
Auditory
Auditory organs
Cochlea - metabolism
Drosophila
Drosophila melanogaster
Drosophila melanogaster - genetics
Drosophila melanogaster - metabolism
Fluorescent Antibody Technique
Hearing Loss, Sensorineural - genetics
Humans
Microscopy, Confocal
Myosin Type II - genetics
Myosin Type II - metabolism
Nonsyndromic hearing loss
Otolaryngology
Pediatrics
Sensory Receptor Cells - metabolism
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Summary:Abstract Objective Human genetic mutations that affect the N-terminal head-domain of the nonmuscle myosin-II (MyoII) molecule can result in nonsyndromic sensorineural hearing loss but the underlying mechanism is unknown. Ultimately, MyoII must be appropriately localized in order to execute endogenous functions. The aim of the current study is to determine whether the head-domain of MyoII regulates in vivo localization of the molecule in living and fixed preparations of the auditory organ. Methods A genetic/transgenic GAL4-UAS approach was used to selectively drive the expression of zip /MyoII ( Drosophila homologue of human nonmuscle MyoII) in Drosophila melanogaster auditory (Johnston's organ) sensory neurons. To follow the distribution of the full-length transgene encoded by MyoII, the N-terminus was fused to green fluorescent protein. Additionally, headless zip /MyoII molecules with and without isoleucine-glutamine or IQ motifs were also expressed in Johnston's organ neurons. Results Removing the entire head domain of MyoII induced localization in neuronal dendrites while removing only a portion of the head but keeping the IQ motif induced localization in the soma and axons of the neurons. Conclusions The findings suggest that the head domain regulates in vivo localization of MyoII in auditory neurons.
ISSN:0165-5876
1872-8464
DOI:10.1016/j.ijporl.2013.02.011