Genetic characterization of the blood-sucking nematodes Libyostrongylus dentatus and Libyostrongylus douglassii supports their different evolutionary history

Libyostrongylus sp. are nematodes that infect ostriches. Libyostrongylus douglassii was first described in ostriches from several countries in the world. Later Libyostrongylus dentatus was morphologically identified in ostriches in the USA and Brazil, and mixed infection is common in the latter coun...

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Bibliographic Details
Published in:Veterinary parasitology 2013-03, Vol.193 (1-3), p.193-199
Main Authors: Andrade, Josiana G., Iñiguez, Alena M., Souza, Adriane N., Marques, Valéria C.L., de Souza Filho, Gonçalo A., Santos, Clóvis P., DaMatta, Renato A.
Format: Article
Language:English
Subjects:
Animals
Base Sequence
Biological Evolution
Bird Diseases - parasitology
DNA, Helminth - genetics
DNA, Ribosomal Spacer - genetics
genes
Genetic diagnosis
genetic distance
genetic techniques and protocols
genetic variation
Internal Transcribed Spacer
internal transcribed spacers
Libyostrongylus dentatus
Libyostrongylus douglassii
mixed infection
Molecular Sequence Data
Nematoda
Nematoda - classification
Nematoda - genetics
Nematodes
ostriches
Phylogeny
ribosomal DNA
Struthio camelus
Struthioniformes
Trichostrongylidae
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Summary:Libyostrongylus sp. are nematodes that infect ostriches. Libyostrongylus douglassii was first described in ostriches from several countries in the world. Later Libyostrongylus dentatus was morphologically identified in ostriches in the USA and Brazil, and mixed infection is common in the latter country. The internal transcribed spacer (ITS) region of the ribosomal DNA gene is used for genetic variability assessment and phylogenetic reconstruction for many organisms. Through genetic analysis the status of different species morphologically defined was confirmed and a molecular method was developed to differentiate both species. ITS1, 5.8S, ITS2 regions of L. douglassii and L. dentatus were characterized. Regarding complete ITS region, the K2-p genetic distance between the species was 0.060 (SE 0.008) and the intra-specific distance was 0.002 (SE 0.001) for L. dentatus and 0.006 (SE 0.002) for L. douglassii. NJ and MP phylogenetic analysis of ITS1 and ITS2 regions indicated that both species belong to the Trichostrongylidae family, and are evolutionarily different, suported by high bootstrap value. Based on ITS DNA polymorphisms, a molecular approach was designed to detect both species. These results are the first molecular characterization of L. douglassii and L. dentatus, and provide new tools for the identification of these parasites of veterinary importance.
ISSN:0304-4017
1873-2550
DOI:10.1016/j.vetpar.2012.11.024