Microbiological profile and antimicrobial susceptibility pattern of infected root canals associated with periapical abscesses

• Published in: European journal of clinical microbiology & infectious diseases 2013-04, Vol.32 (4), p.573-580
• Main Authors: Sousa, E. L. R., Gomes, B. P. F. A., Jacinto, R. C., Zaia, A. A., Ferraz, C. C. R.
• Format: Article
• Language: English
• Subjects: Abscesses; Adolescent; Adult; Anaerobic bacteria; Anaerococcus prevotii; Anti-Bacterial Agents - pharmacology; Antibacterial agents; Antibiotics; Antibiotics. Antiinfectious agents. Antiparasitic agents; Antimicrobial agents; Bacteria; Bacteria, Anaerobic - classification; Bacteria, Anaerobic - drug effects; Bacteria, Anaerobic - isolation & purification; beta-Lactamases - secretion; Biodiversity; Biological and medical sciences; Biomedical and Life Sciences; Biomedicine; Child; Dental Pulp Cavity - microbiology; Dental schools; Dentistry; Endodontics; Female; Humans; Infectious diseases; Internal Medicine; Male; Medical Microbiology; Medical sciences; Microbial Sensitivity Tests; Microorganisms; Middle Aged; Patients; Periapical Abscess - microbiology; Pharmacology. Drug treatments; Young Adult
• ISSN: 0934-9723; 1435-4373
• DOI: 10.1007/s10096-012-1777-5

The aim of this investigation was to identify microorganisms from root canals with periapical abscesses and assess the susceptibility of specific anaerobic bacteria to selected antimicrobials and their β-lactamase production. Sixty root canals were microbiologically investigated. The susceptibility of Anaerococcus prevotii , Fusobacterium necrophorum , F. nucleatum , Parvimonas micra , and Prevotella intermedia / nigrescens to antimicrobials was evaluated with the Etest, whereas β-lactamase production was assessed with nitrocefin. A total of 287 different bacterial strains were recovered, including 201 strict anaerobes. The most frequently strict isolated anaerobes were A. prevotii , P. micra , and F. necrophorum . The selected bacteria were susceptible to all the tested antibiotics, except A. prevotii and Fusobacterium species to azithromycin and erythromycin, as well as A. prevotii and F. necrophorum to metronidazole. None of the microorganisms produced β-lactamase. Gram-positive anaerobic bacteria predominated in the root canals with periapical abscesses. All microorganisms tested were susceptible to benzylpenicillin, amoxicillin, amoxicillin + clavulanate, cefaclor, and clindamycin, producing no β-lactamase.