Cloning and Sequencing of the Spore Germination Gene of Bacillus megaterium ATCC 12872: Similarities to the NaH-Antiporter Gene of Enterococcus hirae

The germination mutant TM-31 of Bacillus megaterium ATCC 12872, was isolated by transposon Tn917 insertional mutagenesis. Glucose, L-proline, L-leucine and KNO3 germinated TM-31 poorly. The DNA in the region of the Tn917 insertion was cloned, and its nucleotide sequence determined. One major open re...

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Bibliographic Details
Published in:MICROBIOLOGY and IMMUNOLOGY 1996, Vol.40(2), pp.99-105
Main Authors: Tani, Katsuji, Watanabe, Takuya, Matsuda, Hideyasu, Nasu, Masao, Kondo, Masaomi
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Bacillus megaterium
Bacillus megaterium - genetics
Bacillus megaterium - isolation & purification
Bacterial Proteins - genetics
Bacterial spore
Bacteriology
Base Sequence
Biological and medical sciences
Cloning, Molecular
DNA Transposable Elements
Enterococcus - genetics
Enterococcus hirae
Fundamental and applied biological sciences. Psychology
Genetics
Germination gene
Membrane Proteins - genetics
Microbiology
Molecular Sequence Data
Mutagenesis, Insertional
Sequence Analysis
Sequence Homology, Amino Acid
Sodium-Hydrogen Exchangers - genetics
Spores, Bacterial - growth & development
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Summary:The germination mutant TM-31 of Bacillus megaterium ATCC 12872, was isolated by transposon Tn917 insertional mutagenesis. Glucose, L-proline, L-leucine and KNO3 germinated TM-31 poorly. The DNA in the region of the Tn917 insertion was cloned, and its nucleotide sequence determined. One major open reading frame was present on the cloned DNA. The hydrophobic protein encoded is presumably membrane-associated. A homology search revealed that the gene encoded in the region of the Tn917 insertion is homologous to napA of Enterococcus hirae. napA codes for the NaH-antiporter. It is hypothesized that transport of cations must play an important role in spore germination in B. megaterium ATCC 12872.
ISSN:0385-5600
1348-0421
DOI:10.1111/j.1348-0421.1996.tb03323.x