Antiplatelet‐derived growth factor (PDGF) activity in the saliva of ixodid ticks is linked with their long mouthparts

Summary The saliva of blood‐feeding arthropods modulates their vertebrate hosts' haemostatic, inflammatory and immune responses to facilitate blood feeding. In a previous study, we showed that salivary gland products from ixodid tick species also manipulate the wound‐healing response by targeti...

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Bibliographic Details
Published in:Parasite immunology 2014-01, Vol.36 (1), p.32-42
Main Authors: Slovák, M., Štibrániová, I., Hajnická, V., Nuttall, P. A.
Format: Article
Language:English
Subjects:
Animals
Arthropoda
Cell Line
Cell Proliferation
Cell Shape
Cytokines - antagonists & inhibitors
Cytokines - metabolism
Female
Fibroblasts - cytology
Growth factor
immune modulation
Ixodidae
Ixodidae - anatomy & histology
Ixodidae - chemistry
Keratinocytes - cytology
Mice
Mouth - anatomy & histology
NIH 3T3 Cells
Platelet-Derived Growth Factor - antagonists & inhibitors
Platelet-Derived Growth Factor - metabolism
Protein Binding
Saliva - chemistry
Salivary Glands - chemistry
tick saliva
Tissue Extracts - pharmacology
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Summary:Summary The saliva of blood‐feeding arthropods modulates their vertebrate hosts' haemostatic, inflammatory and immune responses to facilitate blood feeding. In a previous study, we showed that salivary gland products from ixodid tick species also manipulate the wound‐healing response by targeting at least four different mammalian growth factors: transforming growth factor β1, hepatocyte growth factor, fibroblast growth factor 2 and platelet‐derived growth factor (PDGF). In addition, species that showed PDGF‐binding activity also inhibited cell proliferation in vitro and induced changes in cell morphology accompanied by disruption of the actin cytoskeleton. Here, we show a correlation between the length of the tick hypostome, the sclerotized feeding tube of the mouthparts inserted into the host's skin and anti‐PDGF activity. This apparent link between hypostome length, and hence the potential depth of skin damage, and PDGF‐binding activity was not apparent for the other growth factors or for other cytokines important in wound healing (keratinocyte growth factor, interleukin 6 and stromal cell‐derived factor 1). However, PDGF‐binding activity was no longer correlated with anti‐cell activities, indicating that an additional as yet unidentified activity in tick saliva may affect cellular changes in wound repair.
ISSN:0141-9838
1365-3024
DOI:10.1111/pim.12075