Proteomics analysis of UV-irradiated Lonicera japonica Thunb. with bioactive metabolites enhancement

A previous study showed that the contents of caffeoylquinic acids and iridoids, the major bioactive components in the postharvest Lonicera japonica Thunb., were induced by enhanced ultraviolet (UV)‐A or UV‐B irradiation. To clarify the UV‐responsive key enzymes in the bioactive metabolites biosynthe...

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Bibliographic Details
Published in:Proteomics (Weinheim) 2013-12, Vol.13 (23-24), p.3508-3522
Main Authors: Zhang, Lin, Li, Ximin, Zheng, Wen, Fu, Zhirong, Li, Wenting, Ma, Luyu, Li, Ke, Sun, Lianli, Tian, Jingkui
Format: Article
Language:English
Subjects:
Acids
Biosynthetic pathway
Electrophoresis, Gel, Two-Dimensional
Flowers - metabolism
Flowers - radiation effects
Lonicera - metabolism
Lonicera - radiation effects
Lonicera japonica
Lonicera japonica Thunb
Metabolites
Microbiology
Phenylalanine Ammonia-Lyase - metabolism
Plant Proteins - genetics
Plant Proteins - metabolism
Plant proteomics
Protein Interaction Maps
Proteins
Proteome - genetics
Proteome - metabolism
Proteomics
Secondary metabolites
Stress, Physiological
Ultraviolet Rays
UV irradiation
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Summary:A previous study showed that the contents of caffeoylquinic acids and iridoids, the major bioactive components in the postharvest Lonicera japonica Thunb., were induced by enhanced ultraviolet (UV)‐A or UV‐B irradiation. To clarify the UV‐responsive key enzymes in the bioactive metabolites biosynthetic pathway and the related plant defense mechanism in L. japonica, 2DE in combination with MALDI‐TOF/TOF MS was employed. Seventy‐five out of 196 differential proteins were positively identified. Based on the functions, these proteins were grouped into nine categories, covering a wide range of molecular processes including the secondary metabolites (caffeoylquinic acids and iridoids) biosynthetic‐related proteins, photosynthesis, carbohydrate and energy metabolism, stress, DNA, transport‐related proteins, lipid metabolism, amino acid metabolism, cell wall. Of note is the increasing expression of 1‐deoxy‐d‐xylulose 5‐phosphate reductoisomerase and 5‐enol‐pyruvylshikimate‐phosphate synthase, which was crucial to supply more precursor for the secondary metabolites including caffeoylquinic acids and iridoids. Thus, this study provides both the clues at the protein level for the increase of the two bioactive components upon UV irradiation and the profile of UV‐responsive proteins in L. japonica.
ISSN:1615-9853
1615-9861
DOI:10.1002/pmic.201300212