Genetic assessment of safflower (Carthamus tinctorius L.) collection with microsatellite markers acquired via pyrosequencing method

A genetic evaluation of safflower germplasm collections derived from different geographical regions and countries will provide useful information for sustainable conservation and the utilization of genetic diversity. However, the molecular marker information is limited for evaluation of genetic dive...

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Published in:Molecular ecology resources 2014-01, Vol.14 (1), p.69-78
Main Authors: Lee, Gi-An, Sung, Jung-Sook, Lee, Sok-Young, Chung, Jong-Wook, Yi, Jung-Yoon, Kim, Yeon-Gyu, Lee, Myung-Chul
Format: Article
Language:English
Subjects:
Carthamus tinctorius
Carthamus tinctorius - classification
Carthamus tinctorius - genetics
Carthamus tinctorius L
Cluster Analysis
DNA, Plant - chemistry
DNA, Plant - genetics
Genetic diversity
Genetic markers
Genetic Variation
Genetics, Population
Germplasm
High-Throughput Nucleotide Sequencing
Korea
Microsatellite Repeats
Molecular Sequence Data
Phylogeny
Safflower
Sequence Analysis, DNA
Simple sequence repeat
SSR enrichment
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cited_by cdi_FETCH-LOGICAL-c4426-83f4bf9df35f66ae28ce09c4043402349a637a98e23c38ddd5dcfd27d0aa59df3
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container_title Molecular ecology resources
container_volume 14
creator Lee, Gi-An
Sung, Jung-Sook
Lee, Sok-Young
Chung, Jong-Wook
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Kim, Yeon-Gyu
Lee, Myung-Chul
description A genetic evaluation of safflower germplasm collections derived from different geographical regions and countries will provide useful information for sustainable conservation and the utilization of genetic diversity. However, the molecular marker information is limited for evaluation of genetic diversity of safflower germplasm. In this study, we acquired 509 putative genomic SSR markers for sufficient genome coverage using next‐generation sequencing methods and characterized thirty polymorphic SSRs in safflower collection composed of 100 diverse accessions. The average allele number and expected heterozygosity were 2.8 and 0.386, respectively. Analysis of population structure and phylogeny based on thirty SSR profiles revealed genetic admixture between geographical regions contrary to genetic clustering. However, the accessions from Korea were genetically conserved in distinctive groups in contrast to other safflower gene pool. In conclusion, these new genomic SSRs will facilitate valuable studies to clarify genetic relationships as well as conduct population structure analyses, genetic map construction and association analysis for safflower.
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subjects Carthamus tinctorius
Carthamus tinctorius - classification
Carthamus tinctorius - genetics
Carthamus tinctorius L
Cluster Analysis
DNA, Plant - chemistry
DNA, Plant - genetics
Genetic diversity
Genetic markers
Genetic Variation
Genetics, Population
Germplasm
High-Throughput Nucleotide Sequencing
Korea
Microsatellite Repeats
Molecular Sequence Data
Phylogeny
Safflower
Sequence Analysis, DNA
Simple sequence repeat
SSR enrichment
title Genetic assessment of safflower (Carthamus tinctorius L.) collection with microsatellite markers acquired via pyrosequencing method
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