Whole genome analysis of epizootic hemorrhagic disease virus identified limited genome constellations and preferential reassortment

Epizootic hemorrhagic disease virus (EHDV) is a Culicoides transmitted orbivirus that causes haemorrhagic disease in wild and domestic ruminants. A collection of 44 EHDV isolated from 2008 to 2012 was fully sequenced and analysed phylogenetically. Serotype 2 viruses were the dominant serotype all ye...

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Bibliographic Details
Published in:Journal of general virology 2014-02, Vol.95 (Pt 2), p.434-441
Main Authors: Anbalagan, Srivishnupriya, Cooper, Elyse, Klumper, Pat, Simonson, Randy R, Hause, Ben M
Format: Article
Language:English
Subjects:
Cluster Analysis
Genetic Variation
Genome, Viral
Genotype
Hemorrhagic Disease Virus, Epizootic - genetics
Hemorrhagic Disease Virus, Epizootic - isolation & purification
Humans
Molecular Sequence Data
Phylogeny
Reassortant Viruses - genetics
Recombination, Genetic
Reoviridae Infections - virology
RNA, Viral - genetics
Sequence Analysis, DNA
Sequence Homology
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Description
Summary:Epizootic hemorrhagic disease virus (EHDV) is a Culicoides transmitted orbivirus that causes haemorrhagic disease in wild and domestic ruminants. A collection of 44 EHDV isolated from 2008 to 2012 was fully sequenced and analysed phylogenetically. Serotype 2 viruses were the dominant serotype all years except 2012 when serotype 6 viruses represented 63 % of the isolates. High genetic similarity (>94 % identity) between serotype 1 and 2 virus VP1, VP3, VP4, VP6, NS1, NS2 and NS3 segments prevented identification of reassortment events for these segments. Additionally, there was little genetic diversity (>96 % identity) within serotypes for VP2, VP5 and VP7. Preferential reassortment within the homologous serotype was observed for VP2, VP5 and VP7 segments for type 1 and type 2 viruses. In contrast, type 6 viruses were all reassortants containing VP2 and VP5 derived from an exotic type 6 with the remaining segments most similar to type 2 viruses. These results suggest that reassortment between type 1 and type 2 viruses requires conservation of the VP2, VP5 and VP7 segment constellation while type 6 viruses only require VP2 and VP5 and are restricted to type 2-lineage VP7. As type 6 VP2 and VP5 segments were exclusively identified in viruses with type 2-derived VP7, these results suggest functional complementation between type 2 and type 6 VP7 proteins.
ISSN:0022-1317
1465-2099
DOI:10.1099/vir.0.059659-0