Genome-wide microarray-based analysis of miRNAs expression in patients with acute-on-chronic liver failure

Background Acute-on-chronic liver failure (ACLF) is a severe clinical syndrome that may cause a high mortality. However, the mechanism is still not clear. Characterization of the microRNA (miRNA) profiles in ACLF patients may provide new clues to the pathogenesis and management of this syndrome. Met...

Full description

Saved in:
Bibliographic Details
Published in:Hepatobiliary & pancreatic diseases international 2014-02, Vol.13 (1), p.32-39
Main Authors: Chen, Wen, Yan, Ze-Hui, Wang, Yu-Ming, Xu, Bao-Yan, Deng, Guo-Hong
Format: Article
Language:English
Subjects:
acute-on-chronic liver failure
Acute-On-Chronic Liver Failure - genetics
Acute-On-Chronic Liver Failure - metabolism
Adult
Algorithms
Carrier State
Case-Control Studies
Endocrinology & Metabolism
Female
Gastroenterology and Hepatology
Genome-Wide Association Study
Hepatitis C
Humans
Leukocytes, Mononuclear - metabolism
Male
microarray
microRNA
MicroRNAs - genetics
MicroRNAs - metabolism
Middle Aged
Transcriptome
twins
Twins, Monozygotic - genetics
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Background Acute-on-chronic liver failure (ACLF) is a severe clinical syndrome that may cause a high mortality. However, the mechanism is still not clear. Characterization of the microRNA (miRNA) profiles in ACLF patients may provide new clues to the pathogenesis and management of this syndrome. Methods Genome-wide microarray was performed to compare the different miRNA expression profiles in peripheral blood mononuclear cells of a pair of monozygotic twins, an ACLF patient and an HBV asymptomatic carrier (AsC). The case-control miRNA profiles were compared and confirmed by quantitative reverse transcription-polymerase chain reaction in 104 ACLF patients and 96 AsCs. A combined computational prediction algorithm was used to predict the potential target genes. Results Forty-five miRNAs were increased and eight miRNAs were decreased in the ACLF group. The expressions of hsa-let-7a and hsa-miR-16 were increased by 8.58- and 8.63-fold in ACLF patients compared with that in AsCs, respectively ( P
ISSN:1499-3872
DOI:10.1016/S1499-3872(14)60004-7