Positive prognostic impact of miR-210 in non-small cell lung cancer

• Published in: Lung cancer (Amsterdam, Netherlands) Netherlands), 2014-02, Vol.83 (2), p.272-278
• Main Authors: Eilertsen, Marte, Andersen, Sigve, Al-Saad, Samer, Richardsen, Elin, Stenvold, Helge, Hald, Sigurd M, Al-Shibli, Khalid, Donnem, Tom, Busund, Lill-Tove, Bremnes, Roy M
• Format: Article
• Language: English
• Subjects: Adult; Aged; Aged, 80 and over; Biological and medical sciences; Biomarker; Cancer cells; Carcinoma, Non-Small-Cell Lung - diagnosis; Carcinoma, Non-Small-Cell Lung - genetics; Carcinoma, Non-Small-Cell Lung - mortality; Epithelial Cells - physiology; Female; Gene Expression Regulation, Neoplastic; Hematology, Oncology and Palliative Medicine; Humans; Hypoxia - genetics; Lung Neoplasms - diagnosis; Lung Neoplasms - genetics; Lung Neoplasms - mortality; Male; Medical sciences; MicroRNAs - genetics; MicroRNAs - metabolism; Middle Aged; miR-210; Multiple tumors. Solid tumors. Tumors in childhood (general aspects); Neoplasm Staging; NSCLC; Pneumology; Prognosis; Pulmonary/Respiratory; Stromal Cells - physiology; Survival Analysis; Tissue Array Analysis; Tumor stromal cells; Tumors; Tumors of the respiratory system and mediastinum
• ISSN: 0169-5002; 1872-8332
• DOI: 10.1016/j.lungcan.2013.11.005

Abstract Objectives miR-210 is an important regulator of the cellular response to hypoxia. Therefore, we aimed to explore the prognostic significance of miR-210 in non-small cell lung cancer (NSCLC) patients with stage I-IIIA disease. Materials and methods In addition to clinicopathological and demograpic information, tumor tissues were collected and tissue micro arrays (TMAs) were constructed from 335 patients with stage I-IIIA NSCLC. Expression of miR-210 in cancer cells and stromal cells of the tumor was assessed by in situ hybridization. Results In univariate analyses, high cancer cell ( p = 0.039) and high stromal cell expression ( p = 0.008) of miR-210 were both significantly associated with an improved disease-spesific survival (DSS). High co-expression of miR-210 in cancer and stromal cells was also a positive prognostic factor for DSS ( p = 0.010). In multivariate analysis, miR-210 in stromal cells ( p = 0.011), and miR-210 co-expressed in cancer and stromal cells was an independent prognosticator for DSS ( p = 0.011). Conclusions We show that miR-210 in stromal cells, and co-expressed in cancer cells and stromal cells mediates an independent prognostic impact. It is a candidate marker for prognostic stratification in NSCLC.