The Arabidopsis thaliana RNA Editing Factor SLO2, which Affects the Mitochondrial Electron Transport Chain, Participates in Multiple Stress and Hormone Responses

• Published in: Molecular plant 2014-02, Vol.7 (2), p.290-310
• Main Authors: Zhu, Qiang, Dugardeyn, Jasper, Zhang, Chunyi, Mühlenbock, Per, Eastmond, Peter J., Valcke, Roland, De Coninck, Barbara, Öden, Sevgi, Karampelias, Michael, Cammue, Bruno P.A., Prinsen, Els, Van Der Straeten, Dominique
• Format: Article
• Language: English
• Subjects: ABA; Abscisic Acid - metabolism; Amino acids; Arabidopsis - genetics; Arabidopsis - growth & development; Arabidopsis - metabolism; Arabidopsis - microbiology; Arabidopsis Proteins - genetics; Arabidopsis Proteins - metabolism; Arabidopsis thaliana; Botrytis - physiology; Editing; Electron Transport; ethylene; Ethylenes - metabolism; Gene Expression Regulation, Plant; Marine biology; mitochondria; Mitochondria - genetics; Mitochondria - metabolism; Mitochondrial Proteins - genetics; Mitochondrial Proteins - metabolism; Plant Diseases - microbiology; Plant Growth Regulators - metabolism; plant stress; PPR protein; RNA editing; SLO2; stress; Stress, Physiological
• ISSN: 1674-2052; 1752-9867
• DOI: 10.1093/mp/sst102

Recently, we reported that the novel mitochondrial RNA editing factor SLO2 is essential for mitochondrial electron transport, and vital for plant growth through regulation of carbon and energy metabolism. Here, we show that mutation in SLO2 causes hypersensitivity to ABA and insensitivity to ethylene, suggesting a link with stress responses. Indeed, slo2 mutants are hypersensitive to salt and osmotic stress during the germination stage, while adult plants show increased drought and salt tolerance. Moreover, slo2 mutants are more susceptible to Botrytis cinerea infection. An increased expression of nuclear-encoded stress-responsive genes, as well as mitochondrial-encoded NAD genes of complex I and genes of the alternative respiratory pathway, was observed in slo2 mutants, further enhanced by ABA treatment. In addition, H2O2 accumulation and altered amino acid levels were recorded in slo2 mutants. We conclude that SLO2 is required for plant sensitivity to ABA, ethylene, biotic, and abiotic stress. Although two stress-related RNA editing factors were reported very recently, this study demonstrates a unique role of SLO2, and further supports a link between mitochondrial RNA editing events and stress response. Here, we report that Arabidopsis thaliana RNA editing factor SLO2 participates in multiple stress and hormone responses. Our results support a link between mitochondrial RNA editing events and stress responses.