Influence of prostaglandin F2α analogues on the secretory function of bovine luteal cells and ovarian arterial contractility in vitro

• Published in: The veterinary journal (1997) 2014-01, Vol.199 (1), p.131-137
• Main Authors: Korzekwa, A.J., Lukasik, K., Pilawski, W., Piotrowska-Tomala, K.K., Jaroszewski, J.J., Yoshioka, S., Okuda, K., Skarzynski, D.J.
• Format: Article
• Language: English
• Subjects: Animals; Arterial contraction; Arteries - drug effects; Cattle; Cloprostenol - pharmacology; Corpus luteum; Dinoprost - analogs & derivatives; Dinoprost - pharmacology; Female; Luteal Cells - drug effects; Luteal Cells - physiology; Luteolysis; Ovary - blood supply; PGF2α; Prostaglandin F2α; Prostaglandins F, Synthetic - pharmacology; Vasoconstriction - drug effects
• ISSN: 1090-0233; 1532-2971
• DOI: 10.1016/j.tvjl.2013.09.021

Although prostaglandin (PG) F2α analogues are routinely used for oestrus synchronisation in cattle, their effects on the function of the bovine corpus luteum (CL), and on ovarian arterial contractility, may not reflect the physiological effects of endogenous PGF2α. In the first of two related experiments, the effects of different analogues of PGF2α (aPGF2α) on the secretory function and apoptosis of cultured bovine cells of the CL were assessed. Enzymatically-isolated bovine luteal cells (from between days 8 and 12 of the oestrous cycle), were stimulated for 24h with naturally-occurring PGF2α or aPGF2α (dinoprost, cloprostenol or luprostiol). Secretion of progesterone (P4) was determined and cellular [Ca2+]i mobilisation, as well as cell viability and apoptosis were measured. Naturally-occurring PGF2α and dinoprost stimulated P4 secretion (P