The oncofetal structure of human fibronectin defined by monoclonal antibody FDC-6. Unique structural requirement for the antigen specificity provided by a glycosylhexapeptide

Previously, monoclonal antibody FDC-6 was established, which defines a structure specific for fibronectins isolated from fetal and malignant cells and tissues. The presence of the FDC-6-defined structure at type III connecting segment (III CS) is characteristic of oncofetal fibronectin (onf-FN), and...

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Bibliographic Details
Published in:The Journal of biological chemistry 1988-01, Vol.263 (7), p.3314-3322
Main Authors: Matsuura, H, Takio, K, Titani, K, Greene, T, Levery, S B, Salyan, MEK, Hakomori, S-I
Format: Article
Language:English
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Summary:Previously, monoclonal antibody FDC-6 was established, which defines a structure specific for fibronectins isolated from fetal and malignant cells and tissues. The presence of the FDC-6-defined structure at type III connecting segment (III CS) is characteristic of oncofetal fibronectin (onf-FN), and its absence is characterize of normal fibronectin (nor-FN). Thus, a single glycosylation at a defined threonine residue of the III CS region may induce conformational changes in the peptide to form specific oncofetal epitope recognized by FDC-6 antibody. This finding opens the possibility that a number of other oncofetal epitopes consist of a peptide and a common O-linked carbohydrate and that the combination produces a conformation specific to cancer or a stage of development.
ISSN:0021-9258