Saliva and Serum Levels of B‐Cell Activating Factors and Tumor Necrosis Factor‐α in Patients With Periodontitis

• Published in: Journal of periodontology (1970) 2014-02, Vol.85 (2), p.270-280
• Main Authors: Gümüş, Pınar, Nizam, Nejat, Lappin, David F., Buduneli, Nurcan
• Format: Article
• Language: English
• Subjects: A proliferation inducing ligand protein; Adult; Aggressive Periodontitis - blood; Aggressive Periodontitis - immunology; Alveolar Bone Loss - classification; B-Cell Activating Factor - analysis; B-Cell Activating Factor - blood; B‐cell activating factor; Chronic Periodontitis - blood; Chronic Periodontitis - immunology; Dental Plaque Index; Dentistry; Female; Humans; Interleukin-10 - analysis; Interleukin-10 - blood; Interleukin-6 - analysis; Interleukin-6 - blood; Male; Middle Aged; Periodontal Attachment Loss - classification; Periodontal Index; Periodontal Pocket - classification; periodontitis; Periodontium - immunology; saliva; Saliva - immunology; serum; Smoking - immunology; Tumor Necrosis Factor Ligand Superfamily Member 13 - analysis; Tumor Necrosis Factor Ligand Superfamily Member 13 - blood; Tumor Necrosis Factor-alpha - analysis; Tumor Necrosis Factor-alpha - blood; tumor necrosis factor‐alpha
• ISSN: 0022-3492; 1943-3670
• DOI: 10.1902/jop.2013.130117

Background: B‐lymphocytes play a central and critical role in the adaptive immune response against invading pathogens. This study evaluates saliva and serum levels of APRIL (a proliferation‐inducing ligand), B‐cell activating factor (BAFF), tumor necrosis factor‐α (TNF‐α), interleukin (IL)‐6, and IL‐10 in patients with chronic periodontitis (CP) or aggressive periodontitis (AgP) and periodontally healthy individuals. Methods: Twenty‐five patients with AgP, 20 patients with CP, and 20 periodontally healthy individuals were included. Smoking status was recorded, and all individuals were divided into non‐smokers and smokers. Saliva and serum samples were collected before clinical periodontal measurements. APRIL, BAFF, TNF‐α, IL‐6, and IL‐10 levels in serum and saliva samples were determined by enzyme‐linked immunosorbent assay. Statistical analysis was performed using multivariate analysis of variance and bivariate correlation. Results: Serum and saliva levels of TNF‐α, APRIL, BAFF, IL‐6, and IL‐10 were similar in CP and AgP groups. Serum levels of TNF‐α, APRIL, and BAFF and saliva levels of BAFF were significantly higher in periodontitis groups than healthy controls (P