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Cytokine correlation between sinus tissue and nasal secretions among chronic rhinosinusitis and controls
Objectives/Hypothesis Compare cytokine levels in sinus tissue to sinus secretions from controls and chronic rhinosinusitis patients. Study Design In vitro. Methods Polyurethane foam sponges were placed into middle meati of patients with chronic rhinosinusitis without nasal polyps (CRSsNP), with poly...
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| Published in: | The Laryngoscope 2013-12, Vol.123 (12), p.E72-E78 |
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| Main Authors: | , , , , |
| Format: | Article |
| Language: | English |
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| cites | cdi_FETCH-LOGICAL-c4615-56e48f13c04f2102e6489ab14d3c5173cf759dd60cf2facb1d3fd4e351acc913 |
| container_end_page | E78 |
| container_issue | 12 |
| container_start_page | E72 |
| container_title | The Laryngoscope |
| container_volume | 123 |
| creator | Oyer, Samuel L. Mulligan, Jennifer K. Psaltis, Alkis J. Henriquez, Oswaldo A. Schlosser, Rodney J. |
| description | Objectives/Hypothesis
Compare cytokine levels in sinus tissue to sinus secretions from controls and chronic rhinosinusitis patients.
Study Design
In vitro.
Methods
Polyurethane foam sponges were placed into middle meati of patients with chronic rhinosinusitis without nasal polyps (CRSsNP), with polyps (CRSwNP), and controls. Sinus biopsies were then taken from the same location. Protein levels of tumor necrosis factor‐alpha (TNF‐α), interferon‐γ (IFN‐γ), and interleukins (IL) 2, 4, 6, 8, 10, and 17A were measured via cytometric bead assay for each sample. Protein values from sinus tissue and secretions were compared with Pearson's correlation between samples as well as one‐way ANOVA with posthoc t test between groups.
Results
Samples from 43 patients in total were examined. Mucus was measured from 10 controls, 11 CRSsNP and 10 CRSwNP, and sinus tissue was measured from 10 controls, 15 CRSsNP and 10 CRSwNP. IL‐8 and IFN‐γ levels were outside of the detectable range of the assay. Levels of secreted IL‐2, 4, 6, 10, and 17A correlated with tissue levels (P 0.49) while TNF‐α did not (P = 0.71). CRSsNP had elevated mucus levels of IL‐2, 4, 6, 10, and 17A compared to controls. CRSwNP had elevated mucus levels of IL‐4, 6, 10, 17A, and TNF‐α compared to controls.
Conclusions
Cytokine levels in sinus secretions correlate with levels in sinus tissue and are elevated in CRS versus control based on Th1/Th2 skewing.
Level of Evidence
N/A (in vitro). Laryngoscope, 123:E72–E78, 2013 |
| doi_str_mv | 10.1002/lary.24305 |
| format | article |
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Compare cytokine levels in sinus tissue to sinus secretions from controls and chronic rhinosinusitis patients.
Study Design
In vitro.
Methods
Polyurethane foam sponges were placed into middle meati of patients with chronic rhinosinusitis without nasal polyps (CRSsNP), with polyps (CRSwNP), and controls. Sinus biopsies were then taken from the same location. Protein levels of tumor necrosis factor‐alpha (TNF‐α), interferon‐γ (IFN‐γ), and interleukins (IL) 2, 4, 6, 8, 10, and 17A were measured via cytometric bead assay for each sample. Protein values from sinus tissue and secretions were compared with Pearson's correlation between samples as well as one‐way ANOVA with posthoc t test between groups.
Results
Samples from 43 patients in total were examined. Mucus was measured from 10 controls, 11 CRSsNP and 10 CRSwNP, and sinus tissue was measured from 10 controls, 15 CRSsNP and 10 CRSwNP. IL‐8 and IFN‐γ levels were outside of the detectable range of the assay. Levels of secreted IL‐2, 4, 6, 10, and 17A correlated with tissue levels (P < 0.05 for all, r > 0.49) while TNF‐α did not (P = 0.71). CRSsNP had elevated mucus levels of IL‐2, 4, 6, 10, and 17A compared to controls. CRSwNP had elevated mucus levels of IL‐4, 6, 10, 17A, and TNF‐α compared to controls.
Conclusions
Cytokine levels in sinus secretions correlate with levels in sinus tissue and are elevated in CRS versus control based on Th1/Th2 skewing.
Level of Evidence
N/A (in vitro). Laryngoscope, 123:E72–E78, 2013</description><identifier>ISSN: 0023-852X</identifier><identifier>EISSN: 1531-4995</identifier><identifier>DOI: 10.1002/lary.24305</identifier><identifier>PMID: 23852962</identifier><language>eng</language><publisher>United States: Blackwell Publishing Ltd</publisher><subject>Adult ; Biomarkers - metabolism ; Biopsy ; Chronic Disease ; Chronic rhinosinusitis ; cytokine ; Cytokines ; Cytokines - secretion ; Female ; Humans ; Male ; Middle Aged ; Nasal Mucosa - metabolism ; Nasal Mucosa - pathology ; nasal secretion ; Paranasal Sinuses - metabolism ; Paranasal Sinuses - pathology ; Rhinitis - metabolism ; Rhinitis - pathology ; Severity of Illness Index ; Sinuses ; Sinusitis - metabolism ; Sinusitis - pathology</subject><ispartof>The Laryngoscope, 2013-12, Vol.123 (12), p.E72-E78</ispartof><rights>Copyright © 2013 The American Laryngological, Rhinological and Otological Society, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4615-56e48f13c04f2102e6489ab14d3c5173cf759dd60cf2facb1d3fd4e351acc913</citedby><cites>FETCH-LOGICAL-c4615-56e48f13c04f2102e6489ab14d3c5173cf759dd60cf2facb1d3fd4e351acc913</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23852962$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Oyer, Samuel L.</creatorcontrib><creatorcontrib>Mulligan, Jennifer K.</creatorcontrib><creatorcontrib>Psaltis, Alkis J.</creatorcontrib><creatorcontrib>Henriquez, Oswaldo A.</creatorcontrib><creatorcontrib>Schlosser, Rodney J.</creatorcontrib><title>Cytokine correlation between sinus tissue and nasal secretions among chronic rhinosinusitis and controls</title><title>The Laryngoscope</title><addtitle>The Laryngoscope</addtitle><description>Objectives/Hypothesis
Compare cytokine levels in sinus tissue to sinus secretions from controls and chronic rhinosinusitis patients.
Study Design
In vitro.
Methods
Polyurethane foam sponges were placed into middle meati of patients with chronic rhinosinusitis without nasal polyps (CRSsNP), with polyps (CRSwNP), and controls. Sinus biopsies were then taken from the same location. Protein levels of tumor necrosis factor‐alpha (TNF‐α), interferon‐γ (IFN‐γ), and interleukins (IL) 2, 4, 6, 8, 10, and 17A were measured via cytometric bead assay for each sample. Protein values from sinus tissue and secretions were compared with Pearson's correlation between samples as well as one‐way ANOVA with posthoc t test between groups.
Results
Samples from 43 patients in total were examined. Mucus was measured from 10 controls, 11 CRSsNP and 10 CRSwNP, and sinus tissue was measured from 10 controls, 15 CRSsNP and 10 CRSwNP. IL‐8 and IFN‐γ levels were outside of the detectable range of the assay. Levels of secreted IL‐2, 4, 6, 10, and 17A correlated with tissue levels (P < 0.05 for all, r > 0.49) while TNF‐α did not (P = 0.71). CRSsNP had elevated mucus levels of IL‐2, 4, 6, 10, and 17A compared to controls. CRSwNP had elevated mucus levels of IL‐4, 6, 10, 17A, and TNF‐α compared to controls.
Conclusions
Cytokine levels in sinus secretions correlate with levels in sinus tissue and are elevated in CRS versus control based on Th1/Th2 skewing.
Level of Evidence
N/A (in vitro). Laryngoscope, 123:E72–E78, 2013</description><subject>Adult</subject><subject>Biomarkers - metabolism</subject><subject>Biopsy</subject><subject>Chronic Disease</subject><subject>Chronic rhinosinusitis</subject><subject>cytokine</subject><subject>Cytokines</subject><subject>Cytokines - secretion</subject><subject>Female</subject><subject>Humans</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Nasal Mucosa - metabolism</subject><subject>Nasal Mucosa - pathology</subject><subject>nasal secretion</subject><subject>Paranasal Sinuses - metabolism</subject><subject>Paranasal Sinuses - pathology</subject><subject>Rhinitis - metabolism</subject><subject>Rhinitis - pathology</subject><subject>Severity of Illness Index</subject><subject>Sinuses</subject><subject>Sinusitis - metabolism</subject><subject>Sinusitis - pathology</subject><issn>0023-852X</issn><issn>1531-4995</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><recordid>eNp9kE1vEzEQhi0EoqHlwg9AlrhUSFs8a3s3e6wimoIiQKgqHxfL8c4Stxu7eHbV5t_XSdoeOHAaaeZ5X40ext6AOAEhyg-9TZuTUkmhn7EJaAmFahr9nE3yURZTXf48YK-IroSAWmrxkh2UMm-bqpyw1WwzxGsfkLuYEvZ28DHwJQ63iIGTDyPxwRONyG1oebBke07oEm5B4nYdwx_uVikG73ha-RB3IZ9Du4SLYUixpyP2orM94euHecguzj5ezM6Lxdf5p9nponCqAl3oCtW0A-mE6koQJVZq2tglqFY6nd93Xa2btq2E68rOuiW0smsVSg3WuQbkITve196k-HdEGszak8O-twHjSAZUI-umrqTI6Lt_0Ks4ppCfy1QFCrSYyky931MuRaKEnblJfp2NGxBmq99s9Zud_gy_fagcl2tsn9BH3xmAPXDre9z8p8osTr__eiwt9hlPA949ZWy6NlUta21-fJmb-vxyNv_2-7OZy3tNnqEm</recordid><startdate>201312</startdate><enddate>201312</enddate><creator>Oyer, Samuel L.</creator><creator>Mulligan, Jennifer K.</creator><creator>Psaltis, Alkis J.</creator><creator>Henriquez, Oswaldo A.</creator><creator>Schlosser, Rodney J.</creator><general>Blackwell Publishing Ltd</general><general>Wiley Subscription Services, Inc</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>K9.</scope><scope>7X8</scope></search><sort><creationdate>201312</creationdate><title>Cytokine correlation between sinus tissue and nasal secretions among chronic rhinosinusitis and controls</title><author>Oyer, Samuel L. ; Mulligan, Jennifer K. ; Psaltis, Alkis J. ; Henriquez, Oswaldo A. ; Schlosser, Rodney J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4615-56e48f13c04f2102e6489ab14d3c5173cf759dd60cf2facb1d3fd4e351acc913</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Adult</topic><topic>Biomarkers - metabolism</topic><topic>Biopsy</topic><topic>Chronic Disease</topic><topic>Chronic rhinosinusitis</topic><topic>cytokine</topic><topic>Cytokines</topic><topic>Cytokines - secretion</topic><topic>Female</topic><topic>Humans</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Nasal Mucosa - metabolism</topic><topic>Nasal Mucosa - pathology</topic><topic>nasal secretion</topic><topic>Paranasal Sinuses - metabolism</topic><topic>Paranasal Sinuses - pathology</topic><topic>Rhinitis - metabolism</topic><topic>Rhinitis - pathology</topic><topic>Severity of Illness Index</topic><topic>Sinuses</topic><topic>Sinusitis - metabolism</topic><topic>Sinusitis - pathology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Oyer, Samuel L.</creatorcontrib><creatorcontrib>Mulligan, Jennifer K.</creatorcontrib><creatorcontrib>Psaltis, Alkis J.</creatorcontrib><creatorcontrib>Henriquez, Oswaldo A.</creatorcontrib><creatorcontrib>Schlosser, Rodney J.</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><jtitle>The Laryngoscope</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Oyer, Samuel L.</au><au>Mulligan, Jennifer K.</au><au>Psaltis, Alkis J.</au><au>Henriquez, Oswaldo A.</au><au>Schlosser, Rodney J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cytokine correlation between sinus tissue and nasal secretions among chronic rhinosinusitis and controls</atitle><jtitle>The Laryngoscope</jtitle><addtitle>The Laryngoscope</addtitle><date>2013-12</date><risdate>2013</risdate><volume>123</volume><issue>12</issue><spage>E72</spage><epage>E78</epage><pages>E72-E78</pages><issn>0023-852X</issn><eissn>1531-4995</eissn><abstract>Objectives/Hypothesis
Compare cytokine levels in sinus tissue to sinus secretions from controls and chronic rhinosinusitis patients.
Study Design
In vitro.
Methods
Polyurethane foam sponges were placed into middle meati of patients with chronic rhinosinusitis without nasal polyps (CRSsNP), with polyps (CRSwNP), and controls. Sinus biopsies were then taken from the same location. Protein levels of tumor necrosis factor‐alpha (TNF‐α), interferon‐γ (IFN‐γ), and interleukins (IL) 2, 4, 6, 8, 10, and 17A were measured via cytometric bead assay for each sample. Protein values from sinus tissue and secretions were compared with Pearson's correlation between samples as well as one‐way ANOVA with posthoc t test between groups.
Results
Samples from 43 patients in total were examined. Mucus was measured from 10 controls, 11 CRSsNP and 10 CRSwNP, and sinus tissue was measured from 10 controls, 15 CRSsNP and 10 CRSwNP. IL‐8 and IFN‐γ levels were outside of the detectable range of the assay. Levels of secreted IL‐2, 4, 6, 10, and 17A correlated with tissue levels (P < 0.05 for all, r > 0.49) while TNF‐α did not (P = 0.71). CRSsNP had elevated mucus levels of IL‐2, 4, 6, 10, and 17A compared to controls. CRSwNP had elevated mucus levels of IL‐4, 6, 10, 17A, and TNF‐α compared to controls.
Conclusions
Cytokine levels in sinus secretions correlate with levels in sinus tissue and are elevated in CRS versus control based on Th1/Th2 skewing.
Level of Evidence
N/A (in vitro). Laryngoscope, 123:E72–E78, 2013</abstract><cop>United States</cop><pub>Blackwell Publishing Ltd</pub><pmid>23852962</pmid><doi>10.1002/lary.24305</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0023-852X |
| ispartof | The Laryngoscope, 2013-12, Vol.123 (12), p.E72-E78 |
| issn | 0023-852X 1531-4995 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1493797630 |
| source | Wiley-Blackwell Read & Publish Collection |
| subjects | Adult Biomarkers - metabolism Biopsy Chronic Disease Chronic rhinosinusitis cytokine Cytokines Cytokines - secretion Female Humans Male Middle Aged Nasal Mucosa - metabolism Nasal Mucosa - pathology nasal secretion Paranasal Sinuses - metabolism Paranasal Sinuses - pathology Rhinitis - metabolism Rhinitis - pathology Severity of Illness Index Sinuses Sinusitis - metabolism Sinusitis - pathology |
| title | Cytokine correlation between sinus tissue and nasal secretions among chronic rhinosinusitis and controls |
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