Trehalase Activity in Dormant and Activated Spores of Phycomyces blakesleeanus

Heat treatment of Phycomyces sporangiospores, which breaks dormancy, causes a very rapid 10- to 15fold increase in trehalase activity; soon after the heat shock the enzyme activity decays. This phenomenon can be repeated several times by repeating the heat shocks. Prolonging the heat treatment over...

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Bibliographic Details
Published in:Planta 1972-12, Vol.103 (4), p.327-333
Main Authors: Van Assche, J. A., Carlier, A. R., Dekeersmaeker, Helena I.
Format: Article
Language:English
Subjects:
Aspergillus oryzae
Enzyme activity
Enzymes
Fungal spores
Germination
Heat treatment
Phycomyces
Shock heating
Spore germination
Spores
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Summary:Heat treatment of Phycomyces sporangiospores, which breaks dormancy, causes a very rapid 10- to 15fold increase in trehalase activity; soon after the heat shock the enzyme activity decays. This phenomenon can be repeated several times by repeating the heat shocks. Prolonging the heat treatment over the minimum required time delays the decay of enzyme activity. Cycloheximide does not prevent the rise in enzyme activity. It is suggested that heat treatment converts temporarily an inactive form of trehalase into an active one. Optimal enzyme activity is obtained at pH 7.5 and the enzyme requires metal ions for maximal activity. The possible role of trehalase in the spore-activation process is discussed.
ISSN:0032-0935
1432-2048
DOI:10.1007/BF00386704