Réduction of nitrate by Pseudomonas putrefaciens entrapped in composite agar layer/microporous membrane structures

Composite structures consisting of a Pseudomonas putrefaciens immobilized-cell agar layer bounded by a microporous membrane filter were used for water denitrification. With methanol as the C-source, one litre of high nitrate water (3 mM) was completely freed from NO super(-) sub(3) and NO super(-) s...

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Bibliographic Details
Published in:Biotechnology letters 1988-06, Vol.10 (6), p.399-402
Main Authors: LEMOINE, D, JOUENNE, T, JUNTER, G. A
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biological treatment of waters
Biotechnology
Environment and pollution
Fundamental and applied biological sciences. Psychology
immobilized cells
Industrial applications and implications. Economical aspects
nitrate reduction
Pseudomonas putrefaciens
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Summary:Composite structures consisting of a Pseudomonas putrefaciens immobilized-cell agar layer bounded by a microporous membrane filter were used for water denitrification. With methanol as the C-source, one litre of high nitrate water (3 mM) was completely freed from NO super(-) sub(3) and NO super(-) sub(2) ions in 11 days at a rate of 90 mu mol N-NO super(-) sub(3)/day/g of agar gel, while no production of ammonium ions could be detected. When acetic acid was substituted for methanol, the denitrifying activity was lower. No noticeable contamination of the treated water due to cell leakage from the biocatalytic structures occurred during the incubation periods.
ISSN:0141-5492
1573-6776
DOI:10.1007/BF01087437