The role of epidermal growth factor in prevention of oxidative injury and apoptosis induced by intestinal ischemia/reperfusion in rats

Intestinal ischemia/reperfusion is a major problem which may lead to multiorgan failure and death. The aim of the study was to evaluate the effects of epidermal growth factor (EGF) on apoptosis, cell proliferation, oxidative stress and the antioxidant system in intestinal injury induced by ischemia/...

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Bibliographic Details
Published in:Acta histochemica 2014-01, Vol.116 (1), p.167-175
Main Authors: Arda-Pirincci, Pelin, Bolkent, Sehnaz
Format: Article
Language:English
Subjects:
Animals
Antioxidant enzymes
Apoptosis
Caspase 3 - metabolism
Epidermal growth factor
Epidermal Growth Factor - administration & dosage
Epidermal Growth Factor - physiology
Glutathione - metabolism
Intestinal injury
Ischemia - metabolism
Ischemia - pathology
Ischemia/reperfusion
Jejunum - blood supply
Jejunum - metabolism
Jejunum - pathology
Male
Malondialdehyde - metabolism
Oxidative Stress
Rat
Rats, Sprague-Dawley
Reperfusion Injury - metabolism
Reperfusion Injury - pathology
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Summary:Intestinal ischemia/reperfusion is a major problem which may lead to multiorgan failure and death. The aim of the study was to evaluate the effects of epidermal growth factor (EGF) on apoptosis, cell proliferation, oxidative stress and the antioxidant system in intestinal injury induced by ischemia/reperfusion in rats and to determine if EGF can ameliorate these toxic effects. Intestinal ischemia/reperfusion injury was produced by causing complete occlusion of the superior mesenteric artery for 60min followed by a 60-min reperfusion period. Animals received intraperitoneal injections of 150μg/kg human recombinant EGF 30min prior to the mesenteric ischemia/reperfusion. Mesenteric ischemia/reperfusion caused degeneration of the intestinal mucosa, inhibition of cell proliferation, stimulation of apoptosis and oxidative stress in the small intestine of rats. In the ischemia/reperfusion group, lipid peroxidation was stimulated accompanied by increased intestinal catalase and glutathione peroxidase activities, however, glutathione levels and superoxide dismutase activities were markedly decreased. EGF treatment to rats with ischemia/reperfusion prevented the ischemia/reperfusion-induced oxidative injury by reducing apoptosis and lipid peroxidation, and by increasing antioxidant enzyme activities. These results demonstrate that EGF has beneficial antiapoptotic and antioxidant effects on intestinal injury induced by ischemia/reperfusion in rats.
ISSN:0065-1281
1618-0372
DOI:10.1016/j.acthis.2013.07.005