Carbonic anhydrase IX: A promising diagnostic and prognostic biomarker in breast carcinoma

We examined the expression of carbonic anhydrase IX (CA IX) by immunohistochemical staining using monoclonal antibody M75 (Institute of Virology, Slovak Academy of Sciences, Bratislava) in a group of 38 fibroadenomas and 55 carcinomas of the breast. In each case, the intensity of staining, percentag...

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Bibliographic Details
Published in:Acta histochemica 2014-01, Vol.116 (1), p.89-93
Main Authors: Furjelová, Martina, Kovalská, Mária, Jurková, Katarína, Horáček, Jaroslav, Carbolová, Tereza, Adamkov, Marian
Format: Article
Language:English
Subjects:
Antigens, Neoplasm - metabolism
Biomarkers, Tumor - metabolism
Breast cancer
Breast Neoplasms - enzymology
Breast Neoplasms - pathology
Carbonic Anhydrase IX
Carbonic Anhydrases - metabolism
Female
Fibroadenoma - enzymology
Fibroadenoma - pathology
Humans
Immunohistochemistry
Prognosis
Tumor biomarker
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Summary:We examined the expression of carbonic anhydrase IX (CA IX) by immunohistochemical staining using monoclonal antibody M75 (Institute of Virology, Slovak Academy of Sciences, Bratislava) in a group of 38 fibroadenomas and 55 carcinomas of the breast. In each case, the intensity of staining, percentage of labeled cells and subcellular localization of CA IX were assessed. CA IX was detected in 11/38 fibroadenomas (28.9%). Weak cytoplasmic positivity was dominant in these positive cases. Immunohistochemical analysis of 55 carcinomas showed CA IX expression in 34 cases (61.8%). Membrane staining alone was observed in 27/55 carcinomas (49.1%), while cytoplasmic positivity was found in 4/55 cases (7.3%). Combined membrane and cytoplasmic immunostaining of CA IX was detected in 3/55 carcinomas (5.4%). The intensity of immunoreactivity varied from weak to strong. Under 50% of reactive cells were found in 9/38 fibroadenomas (23.6%) and in 29/55 carcinomas (52.7%). More than 50% of reactive cells were found in 2/38 fibroadenomas (5.3%) and in 5/55 carcinomas (9.1%). Statistical analysis confirmed significant differences in the subcellular localization, intensity of immunoreactivity and percentage of labeled cells in fibroadenomas and carcinomas (p
ISSN:0065-1281
1618-0372
DOI:10.1016/j.acthis.2013.05.009