Combining cell sheet technology and electrospun scaffolding for engineered tubular, aligned, and contractile blood vessels

Abstract Herein we combine cell sheet technology and electrospun scaffolding to rapidly generate circumferentially aligned tubular constructs of human aortic smooth muscles cells with contractile gene expression for use as tissue engineered blood vessel media. Smooth muscle cells cultured on micropa...

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Bibliographic Details
Published in:Biomaterials 2014-03, Vol.35 (9), p.2713-2719
Main Authors: Rayatpisheh, Shahrzad, Heath, Daniel E, Shakouri, Amir, Rujitanaroj, Pim-On, Chew, Sing Yian, Chan-Park, Mary B
Format: Article
Language:English
Subjects:
Acrylamides - pharmacology
Advanced Basic Science
Blood Vessels - physiology
Cells, Cultured
Dentistry
Dimethylpolysiloxanes - chemistry
Dimethylpolysiloxanes - pharmacology
Gene Expression Regulation - drug effects
Humans
Muscle Contraction - drug effects
Muscle Contraction - genetics
Muscle Contraction - physiology
Myocytes, Smooth Muscle - cytology
Myocytes, Smooth Muscle - drug effects
Staining and Labeling
Temperature
Tissue Engineering - methods
Tissue Scaffolds - chemistry
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Summary:Abstract Herein we combine cell sheet technology and electrospun scaffolding to rapidly generate circumferentially aligned tubular constructs of human aortic smooth muscles cells with contractile gene expression for use as tissue engineered blood vessel media. Smooth muscle cells cultured on micropatterned and N-isopropylacrylamide-grafted (pNIPAm) polydimethylsiloxane (PDMS), a small portion of which was covered by aligned electrospun scaffolding, resulted in a single sheet of unidirectionally aligned cells. Upon cooling to room temperature, the scaffold, its adherent cells, and the remaining cell sheet detached and were collected on a mandrel to generating tubular constructs with circumferentially aligned smooth muscle cells which possess contractile gene expression and a single layer of electrospun scaffold as an analogue to a small diameter blood vessel's internal elastic lamina (IEL). This method improves cell sheet handling, results in rapid circumferential alignment of smooth muscle cells which immediately express contractile genes, and introduction of an analogue to small diameter blood vessel IEL.
ISSN:0142-9612
1878-5905
DOI:10.1016/j.biomaterials.2013.12.035