User-Friendly Universal and Durable Subcellular-Scaled Template for Protein Binding: Application to Single-Cell Patterning

• Published in: Advanced functional materials 2013-12, Vol.23 (47), p.5840-5845
• Main Authors: Jang, Jae-Won, Collins, John M., Nettikadan, Saju
• Format: Article
• Language: English
• Subjects: aldehyde-amine reaction; Arrays; cell patterning; dip-pen nanolithography; nanostructures; protein patterning
• ISSN: 1616-301X; 1616-3028
• DOI: 10.1002/adfm.201301088

A new method for subcellular‐sized protein patterning on a SiOx substrate is demonstrated by dip‐pen nanolithography printed aldehyde‐terminated alkylsilane template. The aldehyde‐silane template is stable and durable; for example, subcellular scaled IgG protein array can be obtained using one‐year old aldehyde‐silane template. Moreover, single cell patterning is successfully carried out by extracellular material (ECM) protein microarray and nanoarray fabricated on an aldehyde‐silane template. With more than half of chance, single‐ or double‐cells are successfully attached on fibronectin protein nanoarrays in 21 × 21 μm 2 (7 × 7 dot array) and 42 × 42 μm2 (14 × 14 dot array). The fibronectin nanoarray with small area (21 × 21 μm2) shows the more rate of single cell attachment. Therefore, it is also demonstrated that cell patterning can be controlled by adjusting the nanostructure of ECM materials. A new method for subcellular sized protein patterning on a SiOx substrate is demonstrated by dip‐pen nanolithography printed aldehyde‐terminated alkylsilane template. The aldehyde‐silane template is stable and durable; maintaining its reactivity for up to one year. Subcellular sized arrays of IgG and extracellular matrix (ECM) proteins are obtained by chemical binding (aldehyde‐amine reaction) on the template.