Recent advances in CE-mediated microanalysis for enzyme study

This review gives an overview of the recent developments and applications in the use of CE‐mediated microanalysis for enzyme studies. The period covers mid‐2011 until mid‐2013. Both off‐line and in‐line enzyme assays with their applications using CE are described in this article. For the in‐capillar...

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Bibliographic Details
Published in:Electrophoresis 2014-01, Vol.35 (1), p.119-127
Main Authors: Wang, Xu, Li, Kefeng, Adams, Erwin, Schepdael, Ann Van
Format: Article
Language:English
Subjects:
Bioreactors
Electrophoresis
Electrophoresis, Capillary
Electrophoretically mediated microanalysis
Enzyme Assays
Enzyme study
Enzymes, Immobilized
In-line
Off-line
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Summary:This review gives an overview of the recent developments and applications in the use of CE‐mediated microanalysis for enzyme studies. The period covers mid‐2011 until mid‐2013. Both off‐line and in‐line enzyme assays with their applications using CE are described in this article. For the in‐capillary enzyme reaction, the techniques using electrophoretically mediated microanalysis (EMMA) as well as immobilized enzyme reactor (IMER) are discussed. The applications include the evaluation of enzyme activity, enzyme kinetics, enzyme inhibition, screening of enzyme inhibitors, and the study of enzyme‐mediated drug metabolism.
ISSN:0173-0835
1522-2683
DOI:10.1002/elps.201300294