Singlet oxygen production by human eosinophils

Human eosinophils, stimulated with phorbol myristate acetate, were found to produce 1268 nm chemiluminescence characteristic of singlet oxygen. Singlet oxygen generation required the presence of bromide ion. A bromide ion concentration of 100 microM, comparable to the total bromine content of whole...

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Bibliographic Details
Published in:The Journal of biological chemistry 1988-07, Vol.263 (20), p.9692-9696
Main Authors: Kanofsky, J R, Hoogland, H, Wever, R, Weiss, S J
Format: Article
Language:English
Subjects:
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Bromides - blood
Enzymes and enzyme inhibitors
Eosinophils - drug effects
Eosinophils - metabolism
Fundamental and applied biological sciences. Psychology
Humans
Hydrogen Peroxide - blood
Kinetics
Luminescent Measurements
Oxidoreductases
Oxygen - blood
Peroxidase - blood
Photochemistry
Singlet Oxygen
Spectrophotometry
Tetradecanoylphorbol Acetate - pharmacology
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Summary:Human eosinophils, stimulated with phorbol myristate acetate, were found to produce 1268 nm chemiluminescence characteristic of singlet oxygen. Singlet oxygen generation required the presence of bromide ion. A bromide ion concentration of 100 microM, comparable to the total bromine content of whole blood, was sufficient for the eosinophils to generate measurable amounts of singlet oxygen. For the conditions used (10(7) cells/ml and 10 micrograms/ml phorbol myristate acetate), the duration of the singlet oxygen generation was brief, about 5 min, and the total yield of singlet oxygen was modest, 1.0 +/- 0.1 microM. The cells remained viable after the singlet oxygen production ceased. This is the first demonstration of singlet oxygen production from living cells. The singlet oxygen generated by eosinophils likely results from a peroxidase-catalyzed mechanism, since a purified eosinophil peroxidase-hydrogen peroxide-bromide system was also shown to produce singlet oxygen. The unique properties of eosinophil peroxidase are illustrated by the fact that at p2H 7.0 and with 100 microM bromide, eosinophil peroxidase generated 20 +/- 2% of the theoretical yield of singlet oxygen, whereas under identical conditions, myeloperoxidase and lactoperoxidase produced only 1.0 +/- 0.1% and -0.1 +/- 0.1%, respectively.
ISSN:0021-9258
1083-351X
DOI:10.1016/S0021-9258(19)81573-9