Isolation and partial characterization of an EGF-like growth factor from rat glioma tissue

Two proteins with approximate molecular weight 8 and 12 kilodaltons, possessing the ability to compete with ( super(125)I)EGF for the receptor on A431 cells, were isolated by acid ethanol extraction from rat glioma tissue, induced by ethylnitrosourea. The preparation obtained contained EGF-competing...

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Bibliographic Details
Published in:Biochemistry (Easton) 1987-01, Vol.52 (1), p.86-91
Main Authors: Tarakhovskii, A M, Osipova, LA
Format: Article
Language:English
Subjects:
EGF-like growth factor
growth factors
rats
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Summary:Two proteins with approximate molecular weight 8 and 12 kilodaltons, possessing the ability to compete with ( super(125)I)EGF for the receptor on A431 cells, were isolated by acid ethanol extraction from rat glioma tissue, induced by ethylnitrosourea. The preparation obtained contained EGF-competing activity in an amount of 10 ng-eq EGF per mu g protein and stimulated the phosphorylation of the protein with molecular weight 170 kilodaltons in membranes of NRK cells. On the basis of the data obtained it was concluded that the investigated growth factor belongs to the family of the so-called EGF-like growth factors.
ISSN:0006-2960