Identification of Three Forms of Human Myelin Basic Protein by cDNA Cloning

We have isolated cDNA clones encoding three separate forms of human myelin basic protein (MBP), 21.5, 18.5, and 17.2 kDa, and have determined the nucleotide sequence of each. The three forms share a common sequence but differ by the inclusion of a 26-residue amino acid sequence near the N terminus o...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1986-07, Vol.83 (13), p.4962-4966
Main Authors: Kamholz, John, De Ferra, Francesca, Puckett, Carmie, Lazzarini, Robert
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Amino acids
Animals
Base Sequence
Biological and medical sciences
Biotechnology
Cloning, Molecular
Complementary DNA
DNA
DNA - genetics
Exons
Fundamental and applied biological sciences. Psychology
Gene Expression Regulation
Genes
Genetic engineering
Genetic technics
Humans
Immunosorbent Techniques
Messenger RNA
Methods. Procedures. Technologies
Mice
Molecular cloning
Molecular Weight
Myelin
Myelin Basic Protein - genetics
Myelin Basic Protein - immunology
Open reading frames
Protein isoforms
Proteins
RNA, Messenger - genetics
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Summary:We have isolated cDNA clones encoding three separate forms of human myelin basic protein (MBP), 21.5, 18.5, and 17.2 kDa, and have determined the nucleotide sequence of each. The three forms share a common sequence but differ by the inclusion of a 26-residue amino acid sequence near the N terminus of the 21.5-kDa protein or by the absence of an 11-residue amino acid sequence near the C terminus of the 17.2-kDa protein. The sequences either added to or deleted from the major 18.5-kDa MBP correspond exactly to exons 2 and 5 of the mouse MBP gene, suggesting that the human and mouse genes have similar exon structures. We have also identified the 21.5-kDa human MBP on immunoblots using antisera raised to a peptide encoded by the mouse exon 2 sequence. Southern blotting studies of human genomic DNA reveal a simple pattern consistent with a single human MBP gene. Thus, the three MBP mRNAs are likely to arise from alternative splicing of a primary human MBP transcript. Conservation of the 26 amino acid mouse exon 2 sequence in human MBP suggests an important role for this sequence in myelination.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.83.13.4962