Effect of growth temperature on phospholipid and fatty acid compositions in a psychrotrophic bacterium, Pseudomonas sp. Strain E-3

• Published in: Plant and cell physiology 1987-10, Vol.28 (7), p.1209-1217
• Main Authors: Wada, M. (Hokkaido Univ., Sapporo (Japan). Faculty of Science), Fukunaga, N, Sasaki, S
• Format: Article
• Language: English
• Subjects: ACIDE GRAS; ACIDOS GRASOS; Action of physical and chemical agents on bacteria; Bacteriology; Biological and medical sciences; COMPOSICION; COMPOSITION; FATTY ACIDS; FOSFOLIPIDOS; Fundamental and applied biological sciences. Psychology; Microbiology; PHOSPHATIDE; PHOSPHOLIPIDS; PSEUDOMONAS; TEMPERATURA; TEMPERATURE
• ISSN: 0032-0781; 1471-9053; 1471-9053
• DOI: 10.1093/oxfordjournals.pcp.a077408

The effect of growth temperature on the compositions of phospholipids and fatty acids of the psychrotrophic bacterium Pseudomonas sp. strain E-3 was studied. The composition of phospholipids (phosphatidylethanolamine, phosphatidylglycerol and cardiolipin) did not differ significantly in cells grown at 5°, 15°, and 30°C. Phosphatidylethanolamine (PE) was the most abundant, amounting to over 70% of total phospholipids. When the growth temperature was lowered, palmitoleic [16:1(Δ9)] and cis-vaccenic [18:1(Δ11)] acids increased at the expense of palmitic (16:0) acid, especially in PE. In vivo experiments using [l-14C]16:0 showed that incorporation of radioactivity into 16:1(Δ9) was oxygen-dependent and cerulenin-insensitive, and incorporation into 18:1(Δ11) was cerulenin-sensitive. These results suggest that 16:0 was aerobically desaturated to 16:1(Δ9) and subsequently elongated to 18:1(Δ11). Desaturation activity of 16:0 in the cells grown at 25°C was enhanced by lowering the assay temperature.