Characterization of a Mammalian Smooth Muscle Myosin Heavy Chain cDNA Clone and Its Expression in Various Smooth Muscle Types

A cDNA clone, SMHC-29, encoding the light meromyosin of smooth muscle myosin heavy chain (MHC), was isolated from a rabbit uterus cDNA library constructed in phage λ gt11. This smooth muscle MHC cDNA demonstrates significant nucleotide and amino acid sequence homologies with known sarcomeric MHC gen...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1988-02, Vol.85 (4), p.1047-1051
Main Authors: Nagai, Ryozo, Larson, David M., Periasamy, Muthu
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Amino acids
Animals
Base Sequence
Biological and medical sciences
Biotechnology
Complementary DNA
Dictyostelium
DNA - genetics
Fundamental and applied biological sciences. Psychology
Genetic engineering
Genetic technics
Humans
Messenger RNA
Methods. Procedures. Technologies
Molecular cloning
Molecular Sequence Data
Muscle, Smooth - metabolism
Muscles
Myasthenia gravis
Myosins - biosynthesis
Myosins - genetics
Nematoda
Nucleotides
Organ Specificity
Rabbits
Rats
Recombinant Proteins - biosynthesis
RNA
Sequence Homology, Nucleic Acid
Skeletal muscle
Smooth muscle
Species Specificity
Striated muscle
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Summary:A cDNA clone, SMHC-29, encoding the light meromyosin of smooth muscle myosin heavy chain (MHC), was isolated from a rabbit uterus cDNA library constructed in phage λ gt11. This smooth muscle MHC cDNA demonstrates significant nucleotide and amino acid sequence homologies with known sarcomeric MHC genes from rabbit, rat skeletal, and nematode body wall myosin, and even with nonmuscle MHC gene from a slime mold (Dictyostelium discoideum), suggesting that smooth muscle, striated muscle, and nonmuscle MHC genes diverged from a common ancestor. The deduced amino acid sequences of the smooth muscle light meromyosin show very similar periodic distributions of hydrophobic and charged residues as found for the light meromyosin of striated muscle MHCs together with a high potential for α -helical formation, indicating an α -helical coiled-coil structure for the smooth muscle light meromyosin sequences. Furthermore, S1 nuclease mapping has revealed that this smooth muscle MHC gene for SMHC-29 is specifically expressed in smooth muscles of vascular and nonvascular types but not in the striated muscles or nonmuscle cells.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.85.4.1047