The effects of 780-nm low-level laser therapy on muscle healing process after cryolesion

• Published in: Lasers in medical science 2014-01, Vol.29 (1), p.91-96
• Main Authors: Brunelli, Roberta M., Rodrigues, Natalia C., Ribeiro, Daniel A., Fernandes, Kelly, Magri, Angela, Assis, Lívia, Parizotto, Nivaldo A., Cliquet, Alberto, Renno, Ana Claudia M., Abreu, Daniela C. C.
• Format: Article
• Language: English
• Subjects: Animals; Biomarkers - metabolism; Dentistry; Disease Models, Animal; Histology; Immunohistochemistry; Lasers; Low-Level Light Therapy - methods; Male; Medicine; Medicine & Public Health; Muscle, Skeletal - injuries; Muscle, Skeletal - metabolism; Muscle, Skeletal - radiation effects; Musculoskeletal system; MyoD Protein - metabolism; Myogenin - metabolism; Optical Devices; Optics; Original Article; Pain management; Photonics; Quantum Optics; Rats; Rats, Wistar; Regeneration - radiation effects; Time Factors; Wound Healing - radiation effects
• ISSN: 0268-8921; 1435-604X
• DOI: 10.1007/s10103-013-1277-6

The objective of this study was to assess the effects of 780-nm low-level laser therapy at different periods of 7, 14 and 21 days after cryolesion, including the dose (10 or 50 J/cm 2 ), to promote a better muscle repair evidenced by histopathological and immunohistochemical analyses. Fifty-four male rats were divided into three groups: injured control group (CG)—injured animals without any treatment; injured 780-nm laser-treated group, at 10 J/cm 2 (G10); and injured 780-nm laser-treated group, at 50 J/cm 2 (G50). Each group was divided into three subgroups ( n  = 6): 7, 14 and 21 days post-injury. Histopathological findings revealed better organised muscle fibres in the G10 and G50 during the periods of 7 and 14 days compared to the CG. The G10 and G50 during the 7 days showed a significant reduction ( p