Adiponectin and p53 mRNA in epicardial and subcutaneous fat from heart failure patients

Objective Heart failure (HF) is associated with a pro‐inflammatory state in epicardial fat, but the involved mechanisms are not entirely clear. The aim of our study was to assess the relationship between p53 and adiponectin mRNA in epicardial adipose tissue (EAT) and subcutaneous adipose tissue (SAT...

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Published in:European journal of clinical investigation 2014-01, Vol.44 (1), p.29-37
Main Authors: Agra, Rosa M., Teijeira-Fernández, Elvis, Pascual-Figal, Domingo, Sánchez-Más, Jesús, Fernández-Trasancos, Ángel, González-Juanatey, José R., Eiras, Sonia
Format: Article
Language:English
Subjects:
Adipocytes - drug effects
Adipocytes - metabolism
Adiponectin
Adiponectin - genetics
Adiponectin - metabolism
Adrenergic beta-Agonists - pharmacology
Aged
Aged, 80 and over
epicardial fat
Female
Heart Failure - metabolism
Humans
Intra-Abdominal Fat - cytology
Intra-Abdominal Fat - drug effects
Intra-Abdominal Fat - metabolism
Isoproterenol - pharmacology
Male
Middle Aged
p53
Pericardium
Real-Time Polymerase Chain Reaction
RNA, Messenger - analysis
RNA, Messenger - drug effects
Stromal Cells - metabolism
Subcutaneous Fat - cytology
Subcutaneous Fat - drug effects
Subcutaneous Fat - metabolism
sympathetic activation and heart failure
Tumor Suppressor Protein p53 - drug effects
Tumor Suppressor Protein p53 - genetics
Tumor Suppressor Protein p53 - metabolism
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Summary:Objective Heart failure (HF) is associated with a pro‐inflammatory state in epicardial fat, but the involved mechanisms are not entirely clear. The aim of our study was to assess the relationship between p53 and adiponectin mRNA in epicardial adipose tissue (EAT) and subcutaneous adipose tissue (SAT) in patients with heart failure and its sympathetic regulation. Methods Epicardial adipose tissue and SAT samples were obtained from 63 patients undergoing elective cardiac surgery. EAT and SAT explants culture from seven patients were stimulated with isoprenaline 0·1 or 1 uM for 6 h. p53 and adiponectin mRNA expression was measured in frozen biopsies or explants culture from both fat pads by real‐time polymerase chain reaction (PCR). Results We observed that EAT expressed more p53 mRNA than SAT (1·73 ± 0·07 vs. 1·69 ± 0·04, P 
ISSN:0014-2972
1365-2362
DOI:10.1111/eci.12186