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The Antitumor Effect of a New Docetaxel-Loaded Microbubble Combined with Low-Frequency Ultrasound In Vitro: Preparation and Parameter Analysis
Purpose To develop a novel docetaxel (DOC)-loaded lipid microbubbles (MBs) for achieving target therapy and overcoming the poor water-solubility drawback of DOC. Methods A novel DOC-loaded microbubble (DOC + MB) was prepared by lyophilization and the physicochemical properties including ultrasound c...
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| Published in: | Pharmaceutical research 2013-06, Vol.30 (6), p.1574-1585 |
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| creator | Ren, Shu-Ting Liao, Yi-Ran Kang, Xiao-Ning Li, Yi-Ping Zhang, Hui Ai, Hong Sun, Qiang Jing, Jing Zhao, Xing-Hua Tan, Li-Fang Shen, Xin-Liang Wang, Bing |
| description | Purpose
To develop a novel docetaxel (DOC)-loaded lipid microbubbles (MBs) for achieving target therapy and overcoming the poor water-solubility drawback of DOC.
Methods
A novel DOC-loaded microbubble (DOC + MB) was prepared by lyophilization and the physicochemical properties including ultrasound contrast imaging of the liver were measured. The anti-tumor effect of the DOC + MBs combined with low-frequency ultrasound (LFUS; 0.8Hz, 2.56 W/cm
2
, 50% cycle duty) on the DLD-1 cancer cell line was examined using an MTT assay.
Results
The physicochemical properties of the two tested formats of DOC + MBs (1.0 mg and 1.6 mg) was shown: concentration, (6.74 ± 0.02) × 10
8
bubbles/mL and (8.27 ± 0.15) × 10
8
bubbles/mL; mean size, 3.296 ± 0.004 μm and 3.387 ± 0.005 μm; pH value, 6.67 ± 0.11 and 6.56 ± 0.05; release rate, 3.41% and 12.50%; Zeta potential, −37.95 ± 7.84 mV and −44.35 ± 8.70 mV; and encapsulation efficiency, 54.9 ± 6.21% and 46.3 ± 5.69%, respectively. Compared with SonoVue, the DOC + MBs similarly enhanced the echo signal of the liver imaging. The anti-tumor effect of the DOC + MBs/LFUS group was significantly better than that of DOC alone and that of the normal MBs/LFUS groups.
Conclusions
The self-made DOC + MBs have potential as a new ultrasound contrast agent and drug-loaded microbubble, and can obviously enhance the antitumor effect of DOC under LFUS exposure. |
| doi_str_mv | 10.1007/s11095-013-0996-5 |
| format | article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1496892226</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1349704715</sourcerecordid><originalsourceid>FETCH-LOGICAL-c435t-7534fa251227fe7af6b6a5e36f9dda5b4a8eaa4ecc6eff941fff4107f833a4813</originalsourceid><addsrcrecordid>eNqFkc9u1DAQxiMEotvCA3BBlhASF4Md23HMrVpaqLRADy3iFk2cMU2VxFvb0bIv0WfGq13-CAlxssbzm8_f-CuKZ5y95ozpN5FzZhRlXFBmTEXVg2LBlRbUMPn1YbFgupS01pIfFccx3jLGam7k4-KoFJJrxctFcX91g-R0Sn2aRx_ImXNoE_GOAPmEG_LOW0zwHQe68tBhRz72Nvh2btsBydKPbT_ly02fbsjKb-h5wLsZJ7sl10MKEP08deRiIl_6FPxbchlwDQFS7ycCuXOZixEThuwAhm3s45PikYMh4tPDeVJcn59dLT_Q1ef3F8vTFbVSqES1EtJBmTcotUMNrmorUCgqZ7oOVCuhRgCJ1lbonJHcOSc5064WAmTNxUnxaq-7Dj47jqkZ-2hxGGBCP8eGS1PVpizL6v-okEYzqbnK6Iu_0Fs_h7zajlKV4ZobnSm-p_JPxhjQNevQjxC2DWfNLtdmn2uTc212uTY75ecH5bkdsfs18TPIDLw8ABAtDC7AZPv4m9OSVVzthMo9F3Nr-obhD4v_fP0HNY27XA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1356917197</pqid></control><display><type>article</type><title>The Antitumor Effect of a New Docetaxel-Loaded Microbubble Combined with Low-Frequency Ultrasound In Vitro: Preparation and Parameter Analysis</title><source>Springer Link</source><creator>Ren, Shu-Ting ; Liao, Yi-Ran ; Kang, Xiao-Ning ; Li, Yi-Ping ; Zhang, Hui ; Ai, Hong ; Sun, Qiang ; Jing, Jing ; Zhao, Xing-Hua ; Tan, Li-Fang ; Shen, Xin-Liang ; Wang, Bing</creator><creatorcontrib>Ren, Shu-Ting ; Liao, Yi-Ran ; Kang, Xiao-Ning ; Li, Yi-Ping ; Zhang, Hui ; Ai, Hong ; Sun, Qiang ; Jing, Jing ; Zhao, Xing-Hua ; Tan, Li-Fang ; Shen, Xin-Liang ; Wang, Bing</creatorcontrib><description>Purpose
To develop a novel docetaxel (DOC)-loaded lipid microbubbles (MBs) for achieving target therapy and overcoming the poor water-solubility drawback of DOC.
Methods
A novel DOC-loaded microbubble (DOC + MB) was prepared by lyophilization and the physicochemical properties including ultrasound contrast imaging of the liver were measured. The anti-tumor effect of the DOC + MBs combined with low-frequency ultrasound (LFUS; 0.8Hz, 2.56 W/cm
2
, 50% cycle duty) on the DLD-1 cancer cell line was examined using an MTT assay.
Results
The physicochemical properties of the two tested formats of DOC + MBs (1.0 mg and 1.6 mg) was shown: concentration, (6.74 ± 0.02) × 10
8
bubbles/mL and (8.27 ± 0.15) × 10
8
bubbles/mL; mean size, 3.296 ± 0.004 μm and 3.387 ± 0.005 μm; pH value, 6.67 ± 0.11 and 6.56 ± 0.05; release rate, 3.41% and 12.50%; Zeta potential, −37.95 ± 7.84 mV and −44.35 ± 8.70 mV; and encapsulation efficiency, 54.9 ± 6.21% and 46.3 ± 5.69%, respectively. Compared with SonoVue, the DOC + MBs similarly enhanced the echo signal of the liver imaging. The anti-tumor effect of the DOC + MBs/LFUS group was significantly better than that of DOC alone and that of the normal MBs/LFUS groups.
Conclusions
The self-made DOC + MBs have potential as a new ultrasound contrast agent and drug-loaded microbubble, and can obviously enhance the antitumor effect of DOC under LFUS exposure.</description><identifier>ISSN: 0724-8741</identifier><identifier>EISSN: 1573-904X</identifier><identifier>DOI: 10.1007/s11095-013-0996-5</identifier><identifier>PMID: 23417512</identifier><identifier>CODEN: PHREEB</identifier><language>eng</language><publisher>Boston: Springer US</publisher><subject>Animals ; Antineoplastic Agents - administration & dosage ; Antineoplastic Agents - chemistry ; Antitumor activity ; Biochemistry ; Biological and medical sciences ; Biomedical and Life Sciences ; Biomedical Engineering and Bioengineering ; Biomedicine ; Cancer ; Cell Line, Tumor ; Cell Proliferation - drug effects ; Dogs ; Drug delivery systems ; Drug Delivery Systems - methods ; Drug Stability ; Humans ; Hydrogen-Ion Concentration ; Lipids ; Lipids - administration & dosage ; Lipids - chemistry ; Liver - diagnostic imaging ; Liver - drug effects ; Medical Law ; Medical sciences ; Microbubbles - therapeutic use ; Multiple tumors. Solid tumors. Tumors in childhood (general aspects) ; Pharmaceutical sciences ; Pharmacology. Drug treatments ; Pharmacology/Toxicology ; Pharmacy ; Research Paper ; Solubility ; Taxoids - chemistry ; Taxoids - therapeutic use ; Tumors ; Ultrasonic imaging ; Ultrasonics - methods ; Ultrasonography ; Water - chemistry</subject><ispartof>Pharmaceutical research, 2013-06, Vol.30 (6), p.1574-1585</ispartof><rights>Springer Science+Business Media New York 2013</rights><rights>2014 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c435t-7534fa251227fe7af6b6a5e36f9dda5b4a8eaa4ecc6eff941fff4107f833a4813</citedby><cites>FETCH-LOGICAL-c435t-7534fa251227fe7af6b6a5e36f9dda5b4a8eaa4ecc6eff941fff4107f833a4813</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=27406155$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23417512$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ren, Shu-Ting</creatorcontrib><creatorcontrib>Liao, Yi-Ran</creatorcontrib><creatorcontrib>Kang, Xiao-Ning</creatorcontrib><creatorcontrib>Li, Yi-Ping</creatorcontrib><creatorcontrib>Zhang, Hui</creatorcontrib><creatorcontrib>Ai, Hong</creatorcontrib><creatorcontrib>Sun, Qiang</creatorcontrib><creatorcontrib>Jing, Jing</creatorcontrib><creatorcontrib>Zhao, Xing-Hua</creatorcontrib><creatorcontrib>Tan, Li-Fang</creatorcontrib><creatorcontrib>Shen, Xin-Liang</creatorcontrib><creatorcontrib>Wang, Bing</creatorcontrib><title>The Antitumor Effect of a New Docetaxel-Loaded Microbubble Combined with Low-Frequency Ultrasound In Vitro: Preparation and Parameter Analysis</title><title>Pharmaceutical research</title><addtitle>Pharm Res</addtitle><addtitle>Pharm Res</addtitle><description>Purpose
To develop a novel docetaxel (DOC)-loaded lipid microbubbles (MBs) for achieving target therapy and overcoming the poor water-solubility drawback of DOC.
Methods
A novel DOC-loaded microbubble (DOC + MB) was prepared by lyophilization and the physicochemical properties including ultrasound contrast imaging of the liver were measured. The anti-tumor effect of the DOC + MBs combined with low-frequency ultrasound (LFUS; 0.8Hz, 2.56 W/cm
2
, 50% cycle duty) on the DLD-1 cancer cell line was examined using an MTT assay.
Results
The physicochemical properties of the two tested formats of DOC + MBs (1.0 mg and 1.6 mg) was shown: concentration, (6.74 ± 0.02) × 10
8
bubbles/mL and (8.27 ± 0.15) × 10
8
bubbles/mL; mean size, 3.296 ± 0.004 μm and 3.387 ± 0.005 μm; pH value, 6.67 ± 0.11 and 6.56 ± 0.05; release rate, 3.41% and 12.50%; Zeta potential, −37.95 ± 7.84 mV and −44.35 ± 8.70 mV; and encapsulation efficiency, 54.9 ± 6.21% and 46.3 ± 5.69%, respectively. Compared with SonoVue, the DOC + MBs similarly enhanced the echo signal of the liver imaging. The anti-tumor effect of the DOC + MBs/LFUS group was significantly better than that of DOC alone and that of the normal MBs/LFUS groups.
Conclusions
The self-made DOC + MBs have potential as a new ultrasound contrast agent and drug-loaded microbubble, and can obviously enhance the antitumor effect of DOC under LFUS exposure.</description><subject>Animals</subject><subject>Antineoplastic Agents - administration & dosage</subject><subject>Antineoplastic Agents - chemistry</subject><subject>Antitumor activity</subject><subject>Biochemistry</subject><subject>Biological and medical sciences</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedical Engineering and Bioengineering</subject><subject>Biomedicine</subject><subject>Cancer</subject><subject>Cell Line, Tumor</subject><subject>Cell Proliferation - drug effects</subject><subject>Dogs</subject><subject>Drug delivery systems</subject><subject>Drug Delivery Systems - methods</subject><subject>Drug Stability</subject><subject>Humans</subject><subject>Hydrogen-Ion Concentration</subject><subject>Lipids</subject><subject>Lipids - administration & dosage</subject><subject>Lipids - chemistry</subject><subject>Liver - diagnostic imaging</subject><subject>Liver - drug effects</subject><subject>Medical Law</subject><subject>Medical sciences</subject><subject>Microbubbles - therapeutic use</subject><subject>Multiple tumors. Solid tumors. Tumors in childhood (general aspects)</subject><subject>Pharmaceutical sciences</subject><subject>Pharmacology. Drug treatments</subject><subject>Pharmacology/Toxicology</subject><subject>Pharmacy</subject><subject>Research Paper</subject><subject>Solubility</subject><subject>Taxoids - chemistry</subject><subject>Taxoids - therapeutic use</subject><subject>Tumors</subject><subject>Ultrasonic imaging</subject><subject>Ultrasonics - methods</subject><subject>Ultrasonography</subject><subject>Water - chemistry</subject><issn>0724-8741</issn><issn>1573-904X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><recordid>eNqFkc9u1DAQxiMEotvCA3BBlhASF4Md23HMrVpaqLRADy3iFk2cMU2VxFvb0bIv0WfGq13-CAlxssbzm8_f-CuKZ5y95ozpN5FzZhRlXFBmTEXVg2LBlRbUMPn1YbFgupS01pIfFccx3jLGam7k4-KoFJJrxctFcX91g-R0Sn2aRx_ImXNoE_GOAPmEG_LOW0zwHQe68tBhRz72Nvh2btsBydKPbT_ly02fbsjKb-h5wLsZJ7sl10MKEP08deRiIl_6FPxbchlwDQFS7ycCuXOZixEThuwAhm3s45PikYMh4tPDeVJcn59dLT_Q1ef3F8vTFbVSqES1EtJBmTcotUMNrmorUCgqZ7oOVCuhRgCJ1lbonJHcOSc5064WAmTNxUnxaq-7Dj47jqkZ-2hxGGBCP8eGS1PVpizL6v-okEYzqbnK6Iu_0Fs_h7zajlKV4ZobnSm-p_JPxhjQNevQjxC2DWfNLtdmn2uTc212uTY75ecH5bkdsfs18TPIDLw8ABAtDC7AZPv4m9OSVVzthMo9F3Nr-obhD4v_fP0HNY27XA</recordid><startdate>20130601</startdate><enddate>20130601</enddate><creator>Ren, Shu-Ting</creator><creator>Liao, Yi-Ran</creator><creator>Kang, Xiao-Ning</creator><creator>Li, Yi-Ping</creator><creator>Zhang, Hui</creator><creator>Ai, Hong</creator><creator>Sun, Qiang</creator><creator>Jing, Jing</creator><creator>Zhao, Xing-Hua</creator><creator>Tan, Li-Fang</creator><creator>Shen, Xin-Liang</creator><creator>Wang, Bing</creator><general>Springer US</general><general>Springer</general><general>Springer Nature B.V</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7RV</scope><scope>7TK</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>KB0</scope><scope>M0S</scope><scope>M1P</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>20130601</creationdate><title>The Antitumor Effect of a New Docetaxel-Loaded Microbubble Combined with Low-Frequency Ultrasound In Vitro: Preparation and Parameter Analysis</title><author>Ren, Shu-Ting ; Liao, Yi-Ran ; Kang, Xiao-Ning ; Li, Yi-Ping ; Zhang, Hui ; Ai, Hong ; Sun, Qiang ; Jing, Jing ; Zhao, Xing-Hua ; Tan, Li-Fang ; Shen, Xin-Liang ; Wang, Bing</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c435t-7534fa251227fe7af6b6a5e36f9dda5b4a8eaa4ecc6eff941fff4107f833a4813</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Animals</topic><topic>Antineoplastic Agents - administration & dosage</topic><topic>Antineoplastic Agents - chemistry</topic><topic>Antitumor activity</topic><topic>Biochemistry</topic><topic>Biological and medical sciences</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedical Engineering and Bioengineering</topic><topic>Biomedicine</topic><topic>Cancer</topic><topic>Cell Line, Tumor</topic><topic>Cell Proliferation - drug effects</topic><topic>Dogs</topic><topic>Drug delivery systems</topic><topic>Drug Delivery Systems - methods</topic><topic>Drug Stability</topic><topic>Humans</topic><topic>Hydrogen-Ion Concentration</topic><topic>Lipids</topic><topic>Lipids - administration & dosage</topic><topic>Lipids - chemistry</topic><topic>Liver - diagnostic imaging</topic><topic>Liver - drug effects</topic><topic>Medical Law</topic><topic>Medical sciences</topic><topic>Microbubbles - therapeutic use</topic><topic>Multiple tumors. Solid tumors. Tumors in childhood (general aspects)</topic><topic>Pharmaceutical sciences</topic><topic>Pharmacology. Drug treatments</topic><topic>Pharmacology/Toxicology</topic><topic>Pharmacy</topic><topic>Research Paper</topic><topic>Solubility</topic><topic>Taxoids - chemistry</topic><topic>Taxoids - therapeutic use</topic><topic>Tumors</topic><topic>Ultrasonic imaging</topic><topic>Ultrasonics - methods</topic><topic>Ultrasonography</topic><topic>Water - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ren, Shu-Ting</creatorcontrib><creatorcontrib>Liao, Yi-Ran</creatorcontrib><creatorcontrib>Kang, Xiao-Ning</creatorcontrib><creatorcontrib>Li, Yi-Ping</creatorcontrib><creatorcontrib>Zhang, Hui</creatorcontrib><creatorcontrib>Ai, Hong</creatorcontrib><creatorcontrib>Sun, Qiang</creatorcontrib><creatorcontrib>Jing, Jing</creatorcontrib><creatorcontrib>Zhao, Xing-Hua</creatorcontrib><creatorcontrib>Tan, Li-Fang</creatorcontrib><creatorcontrib>Shen, Xin-Liang</creatorcontrib><creatorcontrib>Wang, Bing</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Nursing & Allied Health Database</collection><collection>Neurosciences Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>AUTh Library subscriptions: ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Pharmaceutical research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ren, Shu-Ting</au><au>Liao, Yi-Ran</au><au>Kang, Xiao-Ning</au><au>Li, Yi-Ping</au><au>Zhang, Hui</au><au>Ai, Hong</au><au>Sun, Qiang</au><au>Jing, Jing</au><au>Zhao, Xing-Hua</au><au>Tan, Li-Fang</au><au>Shen, Xin-Liang</au><au>Wang, Bing</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The Antitumor Effect of a New Docetaxel-Loaded Microbubble Combined with Low-Frequency Ultrasound In Vitro: Preparation and Parameter Analysis</atitle><jtitle>Pharmaceutical research</jtitle><stitle>Pharm Res</stitle><addtitle>Pharm Res</addtitle><date>2013-06-01</date><risdate>2013</risdate><volume>30</volume><issue>6</issue><spage>1574</spage><epage>1585</epage><pages>1574-1585</pages><issn>0724-8741</issn><eissn>1573-904X</eissn><coden>PHREEB</coden><abstract>Purpose
To develop a novel docetaxel (DOC)-loaded lipid microbubbles (MBs) for achieving target therapy and overcoming the poor water-solubility drawback of DOC.
Methods
A novel DOC-loaded microbubble (DOC + MB) was prepared by lyophilization and the physicochemical properties including ultrasound contrast imaging of the liver were measured. The anti-tumor effect of the DOC + MBs combined with low-frequency ultrasound (LFUS; 0.8Hz, 2.56 W/cm
2
, 50% cycle duty) on the DLD-1 cancer cell line was examined using an MTT assay.
Results
The physicochemical properties of the two tested formats of DOC + MBs (1.0 mg and 1.6 mg) was shown: concentration, (6.74 ± 0.02) × 10
8
bubbles/mL and (8.27 ± 0.15) × 10
8
bubbles/mL; mean size, 3.296 ± 0.004 μm and 3.387 ± 0.005 μm; pH value, 6.67 ± 0.11 and 6.56 ± 0.05; release rate, 3.41% and 12.50%; Zeta potential, −37.95 ± 7.84 mV and −44.35 ± 8.70 mV; and encapsulation efficiency, 54.9 ± 6.21% and 46.3 ± 5.69%, respectively. Compared with SonoVue, the DOC + MBs similarly enhanced the echo signal of the liver imaging. The anti-tumor effect of the DOC + MBs/LFUS group was significantly better than that of DOC alone and that of the normal MBs/LFUS groups.
Conclusions
The self-made DOC + MBs have potential as a new ultrasound contrast agent and drug-loaded microbubble, and can obviously enhance the antitumor effect of DOC under LFUS exposure.</abstract><cop>Boston</cop><pub>Springer US</pub><pmid>23417512</pmid><doi>10.1007/s11095-013-0996-5</doi><tpages>12</tpages></addata></record> |
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| subjects | Animals Antineoplastic Agents - administration & dosage Antineoplastic Agents - chemistry Antitumor activity Biochemistry Biological and medical sciences Biomedical and Life Sciences Biomedical Engineering and Bioengineering Biomedicine Cancer Cell Line, Tumor Cell Proliferation - drug effects Dogs Drug delivery systems Drug Delivery Systems - methods Drug Stability Humans Hydrogen-Ion Concentration Lipids Lipids - administration & dosage Lipids - chemistry Liver - diagnostic imaging Liver - drug effects Medical Law Medical sciences Microbubbles - therapeutic use Multiple tumors. Solid tumors. Tumors in childhood (general aspects) Pharmaceutical sciences Pharmacology. Drug treatments Pharmacology/Toxicology Pharmacy Research Paper Solubility Taxoids - chemistry Taxoids - therapeutic use Tumors Ultrasonic imaging Ultrasonics - methods Ultrasonography Water - chemistry |
| title | The Antitumor Effect of a New Docetaxel-Loaded Microbubble Combined with Low-Frequency Ultrasound In Vitro: Preparation and Parameter Analysis |
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