Polymorphism in PGLYRP-1 gene by PCR-RFLP and its association with somatic cell score in Chinese Holstein

• Published in: Research in veterinary science 2013-10, Vol.95 (2), p.508-514
• Main Authors: Wang, H.L., Li, Z.X., Wang, L.J., He, H., Yang, J., Chen, L., Niu, F.B., Liu, Y., Guo, J.Z., Liu, X.L.
• Format: Article
• Language: English
• Subjects: Animals; breeding; Carrier Proteins - genetics; Carrier Proteins - metabolism; Cattle; dairy cattle; Female; Genes; genetic markers; Haplotypes; Holstein; innate immunity; loci; Mastitis; Mastitis, Bovine - genetics; Microbiology; microorganisms; Milk; Milk - cytology; milk yield; PCR-PIRA; PCR-RFLP; peptidoglycans; PGLYRP-1 gene; polymerase chain reaction; Polymerase Chain Reaction - methods; Polymerase Chain Reaction - veterinary; Polymorphism; Polymorphism, Genetic; Polymorphism, Restriction Fragment Length; restriction fragment length polymorphism; SCC; single nucleotide polymorphism; somatic cells; Veterinary medicine
• ISSN: 0034-5288; 1532-2661
• DOI: 10.1016/j.rvsc.2013.06.005

Bovine peptidoglycan recognition protein 1 (PGLYRP-1), an important pattern recognition molecule (PRM) of the innate immune system, is an effector molecule in killing different microorganisms directly. To investigate whether the PGLYRP-1 gene was associated with mastitis and milk production traits in dairy cattle, the polymorphism of this gene was analyzed by PCR-RFLP in a population of 524 Chinese Holstein. A total of ten single nucleotide polymorphism (SNP) loci were identified. The association analysis of single SNP locus showed that T−35A, T−12G and G+102C were significantly associated (P