Functional isoDNA aptamers: modified thrombin binding aptamers with a 2'-5'-linked sugar-phosphate backbone (isoTBA)

The regioisomeric 3'-deoxy-2'-5'-linked thrombin binding DNA aptamers (isoTBAs) were chemically synthesized and their ability to form unimolecular anti-parallel G-quadruplexes in the presence of K(+) ions was evaluated. These modified sequences retain the function of the native thromb...

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Bibliographic Details
Published in:Chemical communications (Cambridge, England) England), 2014-01, Vol.50 (5), p.605-607
Main Authors: Gunjal, Anita D, Fernandes, Moneesha, Erande, Namrata, Rajamohanan, P R, Kumar, Vaijayanti A
Format: Article
Language:English
Subjects:
Aptamers, Nucleotide - chemical synthesis
Aptamers, Nucleotide - chemistry
Aptamers, Nucleotide - metabolism
G-Quadruplexes
Ions - chemistry
Potassium - chemistry
Protein Binding
Stereoisomerism
Sugar Phosphates - chemistry
Sugar Phosphates - metabolism
Thrombin - chemistry
Thrombin - metabolism
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Summary:The regioisomeric 3'-deoxy-2'-5'-linked thrombin binding DNA aptamers (isoTBAs) were chemically synthesized and their ability to form unimolecular anti-parallel G-quadruplexes in the presence of K(+) ions was evaluated. These modified sequences retain the function of the native thrombin binding aptamer (TBA), exhibit better stability against exonuclease and are capable of slowing down the process of blood clotting.
ISSN:1359-7345
1364-548X
DOI:10.1039/c3cc47569g