Effect of Different Manganese Concentrations during in vitro Maturation of Bovine Oocytes on DNA Integrity of Cumulus Cells and Subsequent Embryo Development

Contents Manganese (Mn) is a trace element present in forages and cereals, and its concentration depends on soil status. Manganese deficiency in cattle, goats and ewes not only impairs oestrous cycle but reduces calf birth weight. The achievement of the first oestrus is delayed, and more attempts ar...

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Bibliographic Details
Published in:Reproduction in domestic animals 2013-12, Vol.48 (6), p.905-911
Main Authors: Anchordoquy, JP, Anchordoquy, JM, Sirini, MA, Mattioli, G, Picco, SJ, Furnus, CC
Format: Article
Language:English
Subjects:
Animal reproduction
Animals
Blastocyst - cytology
Blastocyst - physiology
Cattle
Cattle - embryology
Cattle - physiology
Cellular biology
Comet Assay
Culture Media
Cumulus Cells - drug effects
Cumulus Cells - physiology
DNA damage
DNA Damage - drug effects
Dose-Response Relationship, Drug
Embryo Culture Techniques - veterinary
Embryos
Female
Glutathione
Glutathione Disulfide
In Vitro Oocyte Maturation Techniques - veterinary
Manganese - administration & dosage
Manganese - chemistry
Manganese - pharmacology
Oocytes - drug effects
Oocytes - physiology
Trace elements
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Summary:Contents Manganese (Mn) is a trace element present in forages and cereals, and its concentration depends on soil status. Manganese deficiency in cattle, goats and ewes not only impairs oestrous cycle but reduces calf birth weight. The achievement of the first oestrus is delayed, and more attempts are necessary to obtain a successful conception. This study was conducted to investigate the effect of the availability of supplemental Mn during IVM on DNA damage of cumulus cells and total glutathione (GSH) content in oocytes and cumulus cells. The effect of supplementary Mn during IVM on subsequent embryo development was also studied. The results reported here indicate (i) DNA damage in cumulus cells decreased with 0, 2, 5 and 6 ng/ml Mn supplementation during IVM (p 
ISSN:0936-6768
1439-0531
DOI:10.1111/rda.12184