Human plasma cells express granzyme B

While studying the plasma cell (PC) compartment in human tonsils, we identified that immunoglobulin kappa or lambda chain‐expressing PCs are the main cells expressing granzyme B (GrzB). In vitro studies revealed that activated B cells differentiated into GrzB‐expressing PCs when co‐cultured with mac...

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Bibliographic Details
Published in:European journal of immunology 2014-01, Vol.44 (1), p.275-284
Main Authors: Xu, Wei, Narayanan, Priya, Kang, Ning, Clayton, Sandra, Ohne, Yoichiro, Shi, Peiqing, Herve, Marie‐Cecile, Balderas, Robert, Picard, Capucine, Casanova, Jean‐Laurent, Gorvel, Jean‐Pierre, Oh, Sangkon, Pascual, Virginia, Banchereau, Jacques
Format: Article
Language:English
Subjects:
B cells
Cell Differentiation
Cells, Cultured
Coculture Techniques
Gene Expression Regulation
Granzyme B (GrzB)
Granzymes - genetics
Granzymes - metabolism
Humans
Immunoglobulin kappa-Chains - metabolism
Immunoglobulin lambda-Chains - metabolism
Interleukin-15 - immunology
Interleukins - immunology
Lymphocyte Activation
Macrophages - immunology
Palatine Tonsil - cytology
Plasma
Plasma cells
Plasma Cells - enzymology
Rodents
STAT3 Transcription Factor - metabolism
T-Lymphocytes, Helper-Inducer - immunology
Transcriptional Activation
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Summary:While studying the plasma cell (PC) compartment in human tonsils, we identified that immunoglobulin kappa or lambda chain‐expressing PCs are the main cells expressing granzyme B (GrzB). In vitro studies revealed that activated B cells differentiated into GrzB‐expressing PCs when co‐cultured with macrophages and follicular helper T cells. This effect could be reproduced on combined stimulation of IL‐15 (produced by macrophages) and IL‐21 (produced by T follicular helper cells) in a STAT3‐dependent manner. Whereas IL‐21 triggers the transcription of mRNA of GrzB, IL‐15 synergizes the translation of GrzB proteins. The precise role of GrzB in PC biology remains to be understood and studies in mice will not help as their PCs do not express GrzB.
ISSN:0014-2980
1521-4141
DOI:10.1002/eji.201343711