RNA Synthesis and the Germination of Light-Sensitive Lettuce Seeds

The germination of lettuce seeds is inhibited by the nucleotide base analogue 6-methylpurine. RNA synthesis has been measured during imbibition and germination as 32P-phosphate incorporation into RNA species as fractionated by polyacrylamide gel electrophoresis. Seeds were surface sterilized and imb...

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Bibliographic Details
Published in:Planta 1971-03, Vol.97 (1), p.39-49
Main Authors: Frankland, B., Jarvis, B. C., Cherry, J. H.
Format: Article
Language:English
Subjects:
Antibiotics
Electrophoresis
Fractionation
Gels
Germination
Imbibition
Nucleic acids
RNA
Seeds
Sterilization
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Summary:The germination of lettuce seeds is inhibited by the nucleotide base analogue 6-methylpurine. RNA synthesis has been measured during imbibition and germination as 32P-phosphate incorporation into RNA species as fractionated by polyacrylamide gel electrophoresis. Seeds were surface sterilized and imbibed in the presence of various antibiotics. RNA preparations from lettuce seeds were co-electrophoresed with 3H-RNA prepared from bacteria to check for bacterial contamination of the seeds. There is a much higher rate of RNA synthesis in illuminated, germinating seeds as compared to dark, non-germinating seeds. This difference does not develop until after 12 hours of imbibition at 27°, which is the time of onset of germination and radicle growth.
ISSN:0032-0935
1432-2048
DOI:10.1007/BF00388404