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RNA Synthesis and the Germination of Light-Sensitive Lettuce Seeds
The germination of lettuce seeds is inhibited by the nucleotide base analogue 6-methylpurine. RNA synthesis has been measured during imbibition and germination as 32P-phosphate incorporation into RNA species as fractionated by polyacrylamide gel electrophoresis. Seeds were surface sterilized and imb...
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| Published in: | Planta 1971-03, Vol.97 (1), p.39-49 |
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| Main Authors: | , , |
| Format: | Article |
| Language: | English |
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| cited_by | cdi_FETCH-LOGICAL-c309t-930faadb06680a013f26d0f61d01975c27eb4d1fe85418f322771b76aeb927bb3 |
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| cites | cdi_FETCH-LOGICAL-c309t-930faadb06680a013f26d0f61d01975c27eb4d1fe85418f322771b76aeb927bb3 |
| container_end_page | 49 |
| container_issue | 1 |
| container_start_page | 39 |
| container_title | Planta |
| container_volume | 97 |
| creator | Frankland, B. Jarvis, B. C. Cherry, J. H. |
| description | The germination of lettuce seeds is inhibited by the nucleotide base analogue 6-methylpurine. RNA synthesis has been measured during imbibition and germination as 32P-phosphate incorporation into RNA species as fractionated by polyacrylamide gel electrophoresis. Seeds were surface sterilized and imbibed in the presence of various antibiotics. RNA preparations from lettuce seeds were co-electrophoresed with 3H-RNA prepared from bacteria to check for bacterial contamination of the seeds. There is a much higher rate of RNA synthesis in illuminated, germinating seeds as compared to dark, non-germinating seeds. This difference does not develop until after 12 hours of imbibition at 27°, which is the time of onset of germination and radicle growth. |
| doi_str_mv | 10.1007/BF00388404 |
| format | article |
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C. ; Cherry, J. H.</creator><creatorcontrib>Frankland, B. ; Jarvis, B. C. ; Cherry, J. H.</creatorcontrib><description>The germination of lettuce seeds is inhibited by the nucleotide base analogue 6-methylpurine. RNA synthesis has been measured during imbibition and germination as 32P-phosphate incorporation into RNA species as fractionated by polyacrylamide gel electrophoresis. Seeds were surface sterilized and imbibed in the presence of various antibiotics. RNA preparations from lettuce seeds were co-electrophoresed with 3H-RNA prepared from bacteria to check for bacterial contamination of the seeds. There is a much higher rate of RNA synthesis in illuminated, germinating seeds as compared to dark, non-germinating seeds. This difference does not develop until after 12 hours of imbibition at 27°, which is the time of onset of germination and radicle growth.</description><identifier>ISSN: 0032-0935</identifier><identifier>EISSN: 1432-2048</identifier><identifier>DOI: 10.1007/BF00388404</identifier><identifier>PMID: 24493168</identifier><language>eng</language><publisher>Germany: Springer-Verlag</publisher><subject>Antibiotics ; Electrophoresis ; Fractionation ; Gels ; Germination ; Imbibition ; Nucleic acids ; RNA ; Seeds ; Sterilization</subject><ispartof>Planta, 1971-03, Vol.97 (1), p.39-49</ispartof><rights>Springer-Verlag Berlin Heidelberg 1971</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c309t-930faadb06680a013f26d0f61d01975c27eb4d1fe85418f322771b76aeb927bb3</citedby><cites>FETCH-LOGICAL-c309t-930faadb06680a013f26d0f61d01975c27eb4d1fe85418f322771b76aeb927bb3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/23368941$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/23368941$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,777,781,27905,27906,58219,58452</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24493168$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Frankland, B.</creatorcontrib><creatorcontrib>Jarvis, B. 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This difference does not develop until after 12 hours of imbibition at 27°, which is the time of onset of germination and radicle growth.</description><subject>Antibiotics</subject><subject>Electrophoresis</subject><subject>Fractionation</subject><subject>Gels</subject><subject>Germination</subject><subject>Imbibition</subject><subject>Nucleic acids</subject><subject>RNA</subject><subject>Seeds</subject><subject>Sterilization</subject><issn>0032-0935</issn><issn>1432-2048</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1971</creationdate><recordtype>article</recordtype><recordid>eNpFkEtLw0AUhQdRbK1u3CuzFCF655F5LNtiqxAUrK7DJLmxKW1SMxOh_95Iq67u4Z6Ps_gIuWRwxwD0_WQGIIyRII_IkEnBIw7SHJNh_-YRWBEPyJn3K4C-1PqUDLiUVjBlhmTy-jymi10dlugrT11d0D7SObabqnahamralDSpPpYhWmDtq1B9IU0whC5HukAs_Dk5Kd3a48Xhjsj77OFt-hglL_On6TiJcgE2RFZA6VyRgVIGHDBRclVAqVgBzOo45xozWbASTSyZKQXnWrNMK4eZ5TrLxIjc7He3bfPZoQ_ppvI5rteuxqbzKZPWMhYLo3r0do_mbeN9i2W6bauNa3cpg_THWfrvrIevD7tdtsHiD_2V1ANXe2DlQ9P-90IoYyUT324ibgI</recordid><startdate>197103</startdate><enddate>197103</enddate><creator>Frankland, B.</creator><creator>Jarvis, B. 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| fulltext | fulltext |
| identifier | ISSN: 0032-0935 |
| ispartof | Planta, 1971-03, Vol.97 (1), p.39-49 |
| issn | 0032-0935 1432-2048 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1499115386 |
| source | JSTOR-E-Journals; Springer Online Journal Archives |
| subjects | Antibiotics Electrophoresis Fractionation Gels Germination Imbibition Nucleic acids RNA Seeds Sterilization |
| title | RNA Synthesis and the Germination of Light-Sensitive Lettuce Seeds |
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