An Ultrasensitive Nanowire-Transistor Biosensor for Detecting Dopamine Release from Living PC12 Cells under Hypoxic Stimulation

Dopamine (DA) is an important neurotransmitter that is involved in neuronal signal transduction and several critical illnesses. However, the concentration of DA is extremely low in patients and is difficult to detect using existing electrochemical biosensors with detection limits typically around na...

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Bibliographic Details
Published in:Journal of the American Chemical Society 2013-10, Vol.135 (43), p.16034-16037
Main Authors: Li, Bor-Ran, Hsieh, Ying-Jhu, Chen, Yan-Xi, Chung, Ya-Ting, Pan, Chien-Yuan, Chen, Yit-Tsong
Format: Article
Language:English
Subjects:
Animals
Aptamers, Nucleotide
Biosensing Techniques
DNA - chemistry
Dopamine - chemistry
Dopamine - metabolism
Electrochemistry
Equipment Design
Hypoxia - metabolism
Microcomputers
Nanowires
PC12 Cells
Rats
Transistors, Electronic
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Summary:Dopamine (DA) is an important neurotransmitter that is involved in neuronal signal transduction and several critical illnesses. However, the concentration of DA is extremely low in patients and is difficult to detect using existing electrochemical biosensors with detection limits typically around nanomolar levels (∼10–9 M). Here, we developed a nanoelectronic device as a biosensor for ultrasensitive and selective DA detection by modifying DNA-aptamers on a multiple-parallel-connected (MPC) silicon nanowire field-effect transistor (referred to as MPC aptamer/SiNW-FET). Compared with conventional electrochemical methods, the MPC aptamer/SiNW-FET has been demonstrated to improve the limit of DA detection to
ISSN:0002-7863
1520-5126
DOI:10.1021/ja408485m