Investigation of Glucosinolate Profile and Qualitative Aspects in Sprouts and Roots of Horseradish (Armoracia rusticana) Using LC-ESI–Hybrid Linear Ion Trap with Fourier Transform Ion Cyclotron Resonance Mass Spectrometry and Infrared Multiphoton Dissociation

Within the family of Brassicaceae, an important source of glucosinolates (GLSs) is represented by horseradish (Armoracia rusticana P. Gaertner, B. Meyer & Scherbius), cultivated for its roots, which are grated fresh or processed into a sauce and used as a condiment. The characteristic pungent fl...

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Bibliographic Details
Published in:Journal of agricultural and food chemistry 2012-08, Vol.60 (30), p.7474-7482
Main Authors: Agneta, Rosa, Rivelli, Anna Rita, Ventrella, Emanuela, Lelario, Filomena, Sarli, Giulio, Bufo, Sabino Aurelio
Format: Article
Language:English
Subjects:
Armoracia - chemistry
Armoracia rusticana
Biological and medical sciences
Chromatography, Liquid
Cyclotrons
flavor
Food industries
Fourier Analysis
Fundamental and applied biological sciences. Psychology
glucobrassicin
glucoiberin
gluconapin
Glucosinolates - analysis
horseradish
Indoles - analysis
Italy
Mass Spectrometry
plant extracts
Plant Roots - chemistry
roots
sinigrin
Sulfuric Acids - analysis
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Summary:Within the family of Brassicaceae, an important source of glucosinolates (GLSs) is represented by horseradish (Armoracia rusticana P. Gaertner, B. Meyer & Scherbius), cultivated for its roots, which are grated fresh or processed into a sauce and used as a condiment. The characteristic pungent flavor of the root depends on the abundance of the bioactive GLS molecules. In crude plant extracts (sprouts and roots) of an accession of horseradish largely diffused in the Basilicata region (southern Italy), which develops many sprouts and produces white, fiery, and sharp-flavored marketable roots, we characterized the GLS profile by LC-ESI-LTQ-FTICR-MS and IRMPD. In sprouts and roots we identified 16 and 11 GLSs, respectively. We confirmed the presence of sinigrin, 4-hydroxyglucobrassicin, glucobrassicin, gluconasturtin, and 4-methoxyglucobrassicin and identified glucoiberin, gluconapin, glucocochlearin, glucoconringianin, glucosativin, glucoibarin, 5-hydroxyglucobrassicin, glucocapparilinearisin or glucobrassicanapin, glucotropaeolin, and glucoarabishirsutain, not previously characterized in horseradish. Of particular note was the presence of the putative 2-methylsulfonyl-oxo-ethyl-GLS.
ISSN:0021-8561
1520-5118
DOI:10.1021/jf301294h