Comb-Shaped Conjugates Comprising Hydroxypropyl Cellulose Backbones and Low-Molecular-Weight Poly(N-isopropylacryamide) Side Chains for Smart Hydrogels: Synthesis, Characterization, and Biomedical Applications

Hydroxypropyl cellulose (HPC) possesses a lower critical solution temperature (LCST) above 40 °C, while the poly(N-isopropylacrylamide) (P(NIPAAm)) exhibits a LCST of about 32 °C. Herein, comb-shaped copolymer conjugates of HPC backbones and low-molecular-weight P(NIPAAm) side chains (HPC-g-P(NIPAAm...

Full description

Saved in:
Bibliographic Details
Published in:Bioconjugate chemistry 2010-03, Vol.21 (3), p.456-464
Main Authors: Xu, F. J, Zhu, Y, Liu, F. S, Nie, J, Ma, J, Yang, W. T
Format: Article
Language:English
Subjects:
Acrylic Resins - chemistry
Cell Survival - drug effects
Cellulose
Cellulose - analogs & derivatives
Cellulose - chemistry
Chemical bonds
Chemical compounds
Chemical synthesis
Cross-Linking Reagents - chemical synthesis
Cross-Linking Reagents - chemistry
Dextrans - chemistry
Dextrans - pharmacology
Dose-Response Relationship, Drug
HEK293 Cells
Humans
Hydrogels
Hydrogels - chemical synthesis
Hydrogels - chemistry
Isothiocyanates - chemistry
Molecular Weight
Serum Albumin, Bovine - chemistry
Structure-Activity Relationship
Temperature
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Hydroxypropyl cellulose (HPC) possesses a lower critical solution temperature (LCST) above 40 °C, while the poly(N-isopropylacrylamide) (P(NIPAAm)) exhibits a LCST of about 32 °C. Herein, comb-shaped copolymer conjugates of HPC backbones and low-molecular-weight P(NIPAAm) side chains (HPC-g-P(NIPAAm) or HPN) were prepared via atom transfer radical polymerization (ATRP) from the bromoisobutyryl-functionalized HPC biopolymers. By changing the composition ratio of HPC and P(NIPAAm), the LCSTs of HPNs can be adjusted to be lower than the body temperature. The MTT assay from the HEK293 cell line indicated that HPNs possess reduced cytotoxicity. Some of the hydroxyl groups of HPNs were used as cross-linking sites for the preparation of stable HPN hydrogels. In comparison with the HPC hydrogels, the cross-linked HPN hydrogels possess interconnected pore structures and higher swelling ratios. The in vitro release kinetics of fluorescein isothiocyanate-labeled dextran and BSA (or dextran-FITC and BSA-FITC) as model drugs from the hydrogels showed that the HPN hydrogels are suitable for long-term sustained release of macromolecular drugs at body temperature.
ISSN:1043-1802
1520-4812
DOI:10.1021/bc900337p