Reconstitution of OmpF membrane protein on bended lipid bilayers: perforated hexagonal mesophases

Membrane proteins have been reconstituted on lipid bilayers with zero mean-curvature (cubic phases or vesicles). Here we show that reconstitution of pore-forming membrane proteins can also occur on highly curved lipidic bilayers of reverse hexagonal mesophases, for which the mean-curvature is signif...

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Bibliographic Details
Published in:Chemical communications (Cambridge, England) England), 2014-03, Vol.50 (20), p.2642-2645
Main Authors: Zabara, Alexandru, Negrini, Renata, Baumann, Patric, Onaca-Fischer, Ozana, Mezzenga, Raffaele
Format: Article
Language:English
Subjects:
Biological Transport
Lipid Bilayers - chemistry
Membrane Proteins - chemistry
Models, Biological
Porins - chemistry
Water - chemistry
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Summary:Membrane proteins have been reconstituted on lipid bilayers with zero mean-curvature (cubic phases or vesicles). Here we show that reconstitution of pore-forming membrane proteins can also occur on highly curved lipidic bilayers of reverse hexagonal mesophases, for which the mean-curvature is significantly different from zero. We further show that the membrane protein provides unique topological interconnectivities between the aqueous nanochannels, significantly enhancing mesophase transport properties.
ISSN:1359-7345
1364-548X
DOI:10.1039/c3cc49590f