Real-time monitoring of 3T3-L1 preadipocyte differentiation using a commercially available electric cell-substrate impedance sensor system

[Display omitted] •Real-time in vitro monitoring assay of adipogenesis in murine 3T3-L1 cell model.•Impedance based label free cell monitoring.•Detection of morphological changes associated with differentiation. Real-time analysis offers multiple benefits over traditional end point assays. Here, we...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2014-01, Vol.443 (4), p.1245-1250
Main Authors: Kramer, Adam H., Joos-Vandewalle, Julia, Edkins, Adrienne L., Frost, Carminita L., Prinsloo, Earl
Format: Article
Language:English
Subjects:
1-Methyl-3-isobutylxanthine - pharmacology
3T3-L1 Cells
Adipocytes - cytology
Adipocytes - drug effects
Adipogenesis
Adipogenesis - drug effects
Animals
Biosensing Techniques - instrumentation
Biosensing Techniques - methods
Cell Differentiation - drug effects
Computer Systems
Cytological Techniques - instrumentation
Dexamethasone - pharmacology
Electric Impedance
Impedance
Insulin - pharmacology
Mice
Real-time analysis
Thiazolidinediones - pharmacology
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Summary:[Display omitted] •Real-time in vitro monitoring assay of adipogenesis in murine 3T3-L1 cell model.•Impedance based label free cell monitoring.•Detection of morphological changes associated with differentiation. Real-time analysis offers multiple benefits over traditional end point assays. Here, we present a method of monitoring the optimisation of the growth and differentiation of murine 3T3-L1 preadipocytes to adipocytes using the commercially available ACEA xCELLigence Real-Time Cell Analyser Single Plate (RTCA SP) system. Our findings indicate that the ACEA xCELLigence RTCA SP can reproducibly monitor the primary morphological changes in pre- and post-confluent 3T3-L1 fibroblasts induced to differentiate using insulin, dexamethasone, 3-isobutyl-1-methylxanthine and rosiglitazone; and may be a viable primary method of screening compounds for adipogenic factors.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2013.12.123