Validation of a HPLC method for determination of hydroxymethylfurfural in crude palm oil

• Published in: Food chemistry 2014-07, Vol.154, p.102-107
• Main Authors: Ariffin, Abdul Azis, Ghazali, H.M., Kavousi, Parviz
• Format: Article
• Language: English
• Subjects: 5-Hydroxymethylfurfural (HMF); Accuracy; Biological and medical sciences; Chromatography, High Pressure Liquid - methods; Crude palm oil (CPO); Food toxicology; Furaldehyde - analogs & derivatives; Furaldehyde - analysis; Limit of Detection; Medical sciences; Method validation; Palm Oil; Plant Oils - analysis; Precision; Recovery; Toxicology
• ISSN: 0308-8146; 1873-7072
• DOI: 10.1016/j.foodchem.2013.12.082

•The extraction process for 5-hydroxymethylfurfural (HMF) was optimised.•RP-HPLC method was developed for the detection of HMF in crude palm oil.•The method was validated and showed good precision and accuracy.•The presence of 5-hydroxymethylfurfural in crude palm oil was confirmed. For the first time 5-hydroxymethyl-2-furaldehyde (HMF) was separated from crude palm oil (CPO), and its authenticity was determined using an RP-HPLC method. Separation was accomplished with isocratic elution of a mobile phase comprising water and methanol (92:8 v/v) on a Purospher Star RP-18e column (250mm×4.6mm, 5.0μm). The flow rate was adjusted to 1ml/min and detection was performed at 284nm. The method was validated, and results obtained exhibit a good recovery (95.58% to 98.39%). Assessment of precision showed that the relative standard deviations (RSD%) of retention times and peak areas of spiked samples were less than 0.59% and 2.66%, respectively. Further, the limit of detection (LOD) and LOQ were 0.02, 0.05mg/kg, respectively, and the response was linear across the applied ranges. The crude palm oil samples analysed exhibited HMF content less than 2.27mg/kg.