Ischemic preconditioning increased the intestinal stem cell activities in the intestinal crypts in mice

Abstract Background Ischemic preconditioning (IPC) can protect against ischemia–reperfusion injury in the small intestine. Because intestinal stem cells (ISCs) control the recovery and growth of intestinal villi, this study investigated whether IPC had any effects on the activity of ISCs. Materials...

Full description

Saved in:
Bibliographic Details
Published in:The Journal of surgical research 2014-03, Vol.187 (1), p.85-93
Main Authors: Chen, Yun, MD, PhD, Lee, Shih-Hua, MSc, Tsai, Ya-Hui, PhD, Tseng, Sheng-Hong, MD, PhD
Format: Article
Language:English
Subjects:
Animals
beta Catenin - genetics
beta Catenin - metabolism
Biomarkers - metabolism
Glycogen Synthase Kinase 3 - genetics
Glycogen Synthase Kinase 3 - metabolism
Glycogen Synthase Kinase 3 beta
Hypoxia-Inducible Factor 1, alpha Subunit - genetics
Hypoxia-Inducible Factor 1, alpha Subunit - metabolism
Intestinal stem cells
Intestine, Small - blood supply
Intestine, Small - pathology
Intestine, Small - surgery
Ischemic preconditioning
Ischemic Preconditioning - methods
Laparotomy
Mice
Mice, Inbred C57BL
Microvilli - pathology
Organ Culture Techniques
Reperfusion Injury - pathology
Reperfusion Injury - surgery
Reperfusion Injury - therapy
RNA, Messenger - metabolism
Small intestine
Stem cell markers
Stem Cells - cytology
Surgery
Up-Regulation - physiology
Wnt Signaling Pathway - physiology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Abstract Background Ischemic preconditioning (IPC) can protect against ischemia–reperfusion injury in the small intestine. Because intestinal stem cells (ISCs) control the recovery and growth of intestinal villi, this study investigated whether IPC had any effects on the activity of ISCs. Materials and methods The small intestines of mice were treated with IPC, laparotomy only (sham), or no surgery. The crypt fractions were isolated and the characteristics of ISCs among various groups were compared. The regenerative ability and the number of organoids grown from various crypt fractions were compared. The expression of hypoxia-inducible factor-1α (HIF-1α) and the related proteins of the Wnt-/β-catenin pathway in the crypt fractions were studied. Results The IPC group had higher messenger RNA levels of various stem cell markers than the sham group at days 1 and 2 after surgery. The IPC group exhibited greater regenerative activity and more crypt organoids than the sham group ( P  
ISSN:0022-4804
1095-8673
DOI:10.1016/j.jss.2013.10.001