Real-time visualization of 3-D dynamic microscopic objects using optical diffraction tomography

3-D refractive index (RI) distribution is an intrinsic bio-marker for the chemical and structural information about biological cells. Here we develop an optical diffraction tomography technique for the real-time reconstruction of 3-D RI distribution, employing sparse angle illumination and a graphic...

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Bibliographic Details
Published in:Optics express 2013-12, Vol.21 (26), p.32269-32278
Main Authors: Kim, Kyoohyun, Kim, Kyung Sang, Park, Hyunjoo, Ye, Jong Chul, Park, Yongkeun
Format: Article
Language:English
Subjects:
Algorithms
Cell Tracking - instrumentation
Cells, Cultured
Computer Systems
Equipment Design
Equipment Failure Analysis
Erythrocytes - cytology
Humans
Image Enhancement - instrumentation
Image Enhancement - methods
Imaging, Three-Dimensional - instrumentation
Imaging, Three-Dimensional - methods
Refractometry - instrumentation
Refractometry - methods
Reproducibility of Results
Sensitivity and Specificity
Tomography, Optical - instrumentation
Tomography, Optical - methods
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Summary:3-D refractive index (RI) distribution is an intrinsic bio-marker for the chemical and structural information about biological cells. Here we develop an optical diffraction tomography technique for the real-time reconstruction of 3-D RI distribution, employing sparse angle illumination and a graphic processing unit (GPU) implementation. The execution time for the tomographic reconstruction is 0.21 s for 96(3) voxels, which is 17 times faster than that of a conventional approach. We demonstrated the real-time visualization capability with imaging the dynamics of Brownian motion of an anisotropic colloidal dimer and the dynamic shape change in a red blood cell upon shear flow.
ISSN:1094-4087
1094-4087
DOI:10.1364/oe.21.032269