Characterization of novel VIM carbapenemase, VIM-38, and first detection of GES-5 carbapenem-hydrolyzing β-lactamases in Pseudomonas aeruginosa in Turkey

• Published in: Diagnostic microbiology and infectious disease 2014-03, Vol.78 (3), p.292-294
• Main Authors: Iraz, Meryem, Duzgun, Azer Ozad, Cicek, Aysegul Copur, Bonnin, Rémy A, Ceylan, Aysenur, Saral, Aysegul, Nordmann, Patrice, Sandalli, Cemal
• Format: Article
• Language: English
• Subjects: Amino Acid Substitution; Anti-Bacterial Agents - pharmacology; Bacterial Proteins - genetics; beta-Lactamases - genetics; Carbapenemase; Carbapenems - pharmacology; Class 1 integron; Cloning, Molecular; DNA, Bacterial - isolation & purification; Drug Resistance, Multiple, Bacterial - genetics; ESBL; Genes, Bacterial; Humans; Infectious Disease; Internal Medicine; Microbial Sensitivity Tests; Pseudomonas aeruginosa; Pseudomonas aeruginosa - enzymology; Pseudomonas aeruginosa - genetics; Pseudomonas aeruginosa - isolation & purification; Sequence Analysis, DNA; Turkey; β-lactamase
• ISSN: 0732-8893; 1879-0070
• DOI: 10.1016/j.diagmicrobio.2013.12.003

Abstract Pseudomonas aeruginosa isolates were collected form a Turkish hospital. Antimicrobial susceptibility was performed using the Vitek 2 Compact system, and 24 isolates were categorized as multidrug resistant (n = 18), extensively-drug resistant (n = 5), or pan-drug resistant (n = 1). PCR and DNA sequence analysis revealed that 1 strain possessed the blaGES-5 and another carried a novel blaVIM variant, named VIM-38. This new gene exhibited 1 amino acid substitution (Ala265Val) in comparison to its closest variant, VIM-5. Both VIM encoding genes were clones and demonstrated similar susceptibility profile when expressed in identical background. The presence of VIM-38 increases the diversity of carbapenemases in Turkey.